Evidence map›Paper›PMID 27852949›Full record

ArticlePlant physiology2017

Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway.

Lei Liu, Xiaojing Yan, Xiangxiong Kong, Yiqiang Zhao, Zhizhong Gong, Jing Bo Jin, Yan Guo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. SUMOylation in development and neurodegeneration.Development (Cambridge, England) · 2020
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Lei LiuState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.
Xiaojing YanState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.
Xiangxiong KongState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.ORCID 0000-0002-8436-7787
Yiqiang ZhaoState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.
Zhizhong GongState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.ORCID 0000-0001-6551-6014
Jing Bo JinState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and.ORCID 0000-0001-5847-5138
Yan GuoState Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (L.L., X.Y., Y.Z., Z.G., Y.G.); and guoyan@cau.edu.cn.ORCID 0000-0002-6955-8008
Chinese Academy of Sciences · CNChina Agricultural University · CNInstitute of Botany · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The expression of genes with aberrant structure is prevented at both the transcriptional and posttranscriptional regulation levels. Aberrant gene silencing at the posttranscriptional level is well studied; however, it is not well understood how aberrant genes are silenced at the transcriptional level. In this study, through genetic screening a transgenic report line that harbors an aberrant gene (35S-LUC, lacking 3'-untranslated region [3'-UTR]) and lacks luciferase (LUC) activity, we identify that the small ubiquitin-like modifier (SUMO) protease OTS1 gene is required for maintaining the silence of the reporter 35S-LUC and an endogenous mutator-like element MULE-F19G14 at the transcriptional level, which requires DNA-dependent RNA polymerase (Pol) V and DDR complex, but not Pol IV. The increased transcripts in ots1 mutants are terminated by the 3'-UTRs of downstream genes. In addition to ots1 mutations, mutations in several known or putative SUMO proteases and two SUMO E3 ligases, SIZ1 and MMS21, have similar effects on this silencing regulation. Taken together, our results reveal that the enzymes involved in the SUMOylation process restrain aberrant gene transcription by using a downstream gene 3'-UTR, and this regulation requires a functional Pol V-dependent pathway in Arabidopsis (Arabidopsis thaliana).

Indexed as

3' Untranslated RegionsArabidopsisArabidopsis ProteinsATPases Associated with Diverse Cellular ActivitiesCysteine EndopeptidasesDNA-Directed RNA PolymerasesGene Expression Regulation, PlantGene SilencingLigasesMetabolic Networks and PathwaysMutationNuclear ProteinsPlants, Genetically ModifiedSumoylationTranscription Factors3' Untranslated RegionsArabidopsis ProteinsATPases Associated with Diverse Cellular ActivitiesCysteine EndopeptidasesDNA-Directed RNA PolymerasesDRD1 protein, ArabidopsisLigasesMMS21 protein, ArabidopsisMOM protein, ArabidopsisNuclear ProteinsOTS1 protein, ArabidopsisOTS2 protein, ArabidopsisRNA polymerase V, ArabidopsisSIZ1 protein, ArabidopsisTranscription Factors

Identifiers

PMID27852949
PMCPMC5210737
OpenAlexW2550750468

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.