ArticleMolecular and cellular biology1989
A protein kinase C cDNA without the regulatory domain is active after transfection in vivo in the absence of phorbol ester.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 102 citations in OpenAlex.
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- Thermogenic activation induces FGF21 expression and release in brown adipose tissue.The Journal of biological chemistry · 2011Article
- Functional characterization of the human phosphodiesterase 7A1 promoter.The Biochemical journal · 2003Article
- LKB1, a novel serine/threonine protein kinase and potential tumour suppressor, is phosphorylated by cAMP-dependent protein kinase (PKA) and prenylated in vivo.The Biochemical journal · 2000Article
- An activated protein kinase C alpha gives a differentiation signal for hematopoietic progenitor cells and mimicks macrophage colony-stimulating factor-stimulated signaling events.The Journal of cell biology · 1998Article
- Expression of protein kinase C beta in the heart causes hypertrophy in adult mice and sudden death in neonates.The Journal of clinical investigation · 1997Article
- Role of diacylglycerol-regulated protein kinase C isotypes in growth factor activation of the Raf-1 protein kinase.Molecular and cellular biology · 1997Article
- Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Protein kinase C chimeras: catalytic domains of alpha and beta II protein kinase C contain determinants for isotype-specific function.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Calcium/calmodulin-dependent protein kinase II downregulates both calcineurin and protein kinase C-mediated pathways for cytokine gene transcription in human T cells.The Journal of experimental medicine · 1995Article
- PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system.The Journal of cell biology · 1995Article
- Fibroblast growth factor inhibits MRF4 activity independently of the phosphorylation status of a conserved threonine residue within the DNA-binding domain.Molecular and cellular biology · 1993Article
- NPK1, a tobacco gene that encodes a protein with a domain homologous to yeast BCK1, STE11, and Byr2 protein kinases.Molecular and cellular biology · 1993Article
- Yeast phenotype classifies mammalian protein kinase C cDNA mutants.Molecular and cellular biology · 1993Article
- Protein kinase C isoenzymes: divergence in signal transduction?The Biochemical journal · 1993Review
- Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes.Molecular and cellular biology · 1992Article
- An 11-base-pair DNA sequence motif apparently unique to the human interleukin 4 gene confers responsiveness to T-cell activation signals.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Transcriptional activation of the cardiac myosin light chain 2 and atrial natriuretic factor genes by protein kinase C in neonatal rat ventricular myocytes.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Increased expression and DNA-binding activity of transcription factor Sp1 in doxorubicin-resistant HL-60 leukemia cells.Molecular and cellular biology · 1990Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We constructed mutant protein kinase C (PKC) cDNAs which expressed PKC activity in vivo in the absence of phorbol ester activation. A hybrid PKC gene, PKAC, was constructed by substituting the coding region for the N-terminal 253 amino acids of PKC alpha with the N-terminal 17 amino acids of the cyclic AMP-dependent protein kinase catalytic subunit (PKA). A truncated PKC gene, delta PKC beta, lacking the coding region for amino acid positions 6 to 159 of PKC beta was also constructed. These mutant kinase genes expressed under the control of the SR alpha promoter activated the c-fos gene enhancer in Jurkat cells and initiated maturation of Xenopus laevis oocytes. Phorbol ester binding activity was absent in both constructs but was preserved in another hybrid gene, PKCA, which was composed of the coding region for 1 to 253 amino acids of PKC alpha at the N-terminal side and the coding region for 18 to 350 amino acids of PKA at the C-terminal side. These results indicate that elimination of the regulatory domain of PKC produces constitutively active PKC that can bypass activation by the phorbol ester. delta PKC beta, in synergy with a calcium ionophore, was capable of activating the interleukin 2 promoter, indicating that cooperation of PKC-dependent and calcium-dependent pathways is necessary for activation of the interleukin 2 gene.
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