ArticleBMC biotechnology2016
A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum.
Article in BMC biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Horizontal gene transfer in Saccharomyces cerevisiae and other Saccharomycotina yeasts: a review.World journal of microbiology & biotechnology · 2026Review
- Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption.Plant molecular biology · 2024Article
- Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs.FEMS microbiology ecology · 2024Article
- Beyond rational-biosensor-guided isolation of 100 independently evolved bacterial strain variants and comparative analysis of their genomes.BMC biology · 2023Article
- Genome-Wide Analysis and Functional Characterization of Pyruvate Kinase (PK) Gene Family Modulating Rice Yield and Quality.International journal of molecular sciences · 2022Article
- Article
- An Integrated Database of Small RNAs and Their Interplay With Transcriptional Gene Regulatory Networks in Corynebacteria.Frontiers in microbiology · 2021Article
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
- Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium.Scientific reports · 2017Article
- A new genome-scale metabolic model ofBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involved in the tricarboxylic acid cycle, anaplerosis and energy anabolism, which places Pyk at a primary metabolic intersection. Pyks are highly conserved in most bacteria and lower eukaryotes. Corynebacterium glutamicum is an industrial workhorse for the production of various amino acids and organic acids. Although C. glutamicum was assumed to possess only one Pyk (pyk1, NCgl2008), NCgl2809 was annotated as a pyruvate kinase with an unknown role.
resultsHere, we identified that NCgl2809 was a novel pyruvate kinase (pyk2) in C. glutamicum. Complementation of the WTΔpyk1Δpyk2 strain with the pyk2 gene restored its growth on D-ribose, which demonstrated that Pyk2 could substitute for Pyk1 in vivo. Pyk2 was co-dependent on Mn
conclusionsPyk2 functioned as a pyruvate kinase and contributed to the increased level of Pyk activity under oxygen deprivation.
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