ArticleBMC cell biology2016
A functional endosomal pathway is necessary for lysosome biogenesis in Drosophila.
Article in BMC cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- IntegratingFrontiers in pharmacology · 2025Article
- Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation.eLife · 2024Article
- AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in neurodevelopmental disorders.Disease models & mechanisms · 2023Article
- Role of Rab5 early endosomes in regulatingiScience · 2023Article
- Article
- AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorder.bioRxiv : the preprint server for biology · 2023Article
- Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes.The Journal of cell biology · 2023Article
- The ESCRT-III ProteinMedical sciences (Basel, Switzerland) · 2022Article
- The ubiquitin-specific protease 8 antagonizes melatonin-induced endocytic degradation of MTJournal of advanced research · 2022Article
- Article
- Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.Journal of extracellular vesicles · 2022Article
- Exploiting the Potential ofBiomedicines · 2021Review
- The Role of Secretory Pathways inJournal of fungi (Basel, Switzerland) · 2020Review
- Ubiquitin-specific protease 8 (USP8/UBPy): a prototypic multidomain deubiquitinating enzyme with pleiotropic functions.Biochemical Society transactions · 2019Review
- Review
- CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis.PLoS genetics · 2018Article
- Mitofusin-Dependent ER Stress Triggers Glial Dysfunction and Nervous System Degeneration in aFrontiers in molecular neuroscience · 2018Article
- Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLysosomes are the major catabolic compartment within eukaryotic cells, and their biogenesis requires the integration of the biosynthetic and endosomal pathways. Endocytosis and autophagy are the primary inputs of the lysosomal degradation pathway. Endocytosis is specifically needed for the degradation of membrane proteins whereas autophagy is responsible for the degradation of cytoplasmic components. We previously identified the deubiquitinating enzyme UBPY/USP8 as being necessary for lysosomal biogenesis and productive autophagy in Drosophila. Because UBPY/USP8 has been widely described for its function in the endosomal system, we hypothesized that disrupting the endosomal pathway itself may affect the biogenesis of the lysosomes.
resultsIn the present study, we blocked the progression of the endosomal pathway at different levels of maturation of the endosomes by expressing in fat body cells either dsRNAs or dominant negative mutants targeting components of the endosomal machinery: Shibire, Rab4, Rab5, Chmp1 and Rab7. We observed that inhibition of endosomal trafficking at different steps in vivo is systematically associated with defects in lysosome biogenesis, resulting in autophagy flux blockade.
conclusionOur results show that the integrity of the endosomal system is required for lysosome biogenesis and productive autophagy in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.