ArticleBiophysical journal2016
Segmentation of 3D Trajectories Acquired by TSUNAMI Microscope: An Application to EGFR Trafficking.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imaging.Cell · 2026Article
- Boosting microparticle tracking with neuromorphic cameras by optical modulation.Scientific reports · 2025Article
- Trajectory Analysis in Single-Particle Tracking: From Mean Squared Displacement to Machine Learning Approaches.International journal of molecular sciences · 2024Review
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- An Estimation Algorithm for General Linear Single Particle Tracking Models with Time-Varying Parameters.Molecules (Basel, Switzerland) · 2021Article
- Autophosphorylation of EGFR at Y954 Facilitated Homodimerization and Enhanced Downstream Signals.Biophysical journal · 2020Article
- Article
- Short Trajectory Segmentation With 1D Unet Framework: Application To Secretory Vesicle Dynamics.Proceedings. IEEE International Symposium on Biomedical Imaging · 2020Article
- High-Precision Protein-Tracking With Interferometric Scattering Microscopy.Frontiers in cell and developmental biology · 2020Article
- Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer.Cancers · 2019Article
- Measuring DNA Hybridization Kinetics in Live Cells Using a Time-Resolved 3D Single-Molecule Tracking Method.Journal of the American Chemical Society · 2019Article
- Two-Dimensional and Three-Dimensional Single Particle Tracking of Upconverting Nanoparticles in Living Cells.International journal of molecular sciences · 2019Review
- Assessing metastatic potential of breast cancer cells based on EGFR dynamics.Scientific reports · 2019Article
- Identifying diffusive motions in single-particle trajectories on the plasma membrane via fractional time-series models.Physical review. E · 2019Article
- Imaging, Tracking and Computational Analyses of Virus Entry and Egress with the Cytoskeleton.Viruses · 2018Review
- Multistep Track Segmentation and Motion Classification for Transient Mobility Analysis.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Whereas important discoveries made by single-particle tracking have changed our view of the plasma membrane organization and motor protein dynamics in the past three decades, experimental studies of intracellular processes using single-particle tracking are rather scarce because of the lack of three-dimensional (3D) tracking capacity. In this study we use a newly developed 3D single-particle tracking method termed TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) to investigate epidermal growth factor receptor (EGFR) trafficking dynamics in live cells at 16/43 nm (xy/z) spatial resolution, with track duration ranging from 2 to 10 min and vertical tracking depth up to tens of microns. To analyze the long 3D trajectories generated by the TSUNAMI microscope, we developed a trajectory analysis algorithm, which reaches 81% segment classification accuracy in control experiments (termed simulated movement experiments). When analyzing 95 EGF-stimulated EGFR trajectories acquired in live skin cancer cells, we find that these trajectories can be separated into three groups-immobilization (24.2%), membrane diffusion only (51.6%), and transport from membrane to cytoplasm (24.2%). When EGFRs are membrane-bound, they show an interchange of Brownian diffusion and confined diffusion. When EGFRs are internalized, transitions from confined diffusion to directed diffusion and from directed diffusion back to confined diffusion are clearly seen. This observation agrees well with the model of clathrin-mediated endocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.