ArticlePloS one2016
Transcriptional Responses and Gentiopicroside Biosynthesis in Methyl Jasmonate-Treated Gentiana macrophylla Seedlings.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Defining Phytochemical Metabolomes of Somatic HybridsMolecules (Basel, Switzerland) · 2025Article
- Tissue-specific biosynthesis and regulation of alkaloids, flavonoids, and terpenoids in fenugreek (Frontiers in plant science · 2025Article
- Genomic characterization of WRKY transcription factors related to secoiridoid biosynthesis in Gentiana macrophylla.BMC plant biology · 2024Article
- Monoterpene glucosides in Eustoma grandiflorum roots promote hyphal branching in arbuscular mycorrhizal fungi.Plant physiology · 2023Article
- Functional iridoid synthases from iridoid producing and non-producingFrontiers in plant science · 2023Article
- De Novo Transcriptome Analysis Reveals Flowering-Related Genes That Potentially Contribute to Flowering-Time Control in the Japanese Cultivated GentianInternational journal of molecular sciences · 2022Article
- Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview.Frontiers in plant science · 2022Review
- Methyl Jasmonate Affects Photosynthesis Efficiency, Expression ofInternational journal of molecular sciences · 2020Article
- Secoiridoids Metabolism Response to Wounding in Common Centaury (Plants (Basel, Switzerland) · 2019Article
- Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway.Annals of translational medicine · 2019Article
- Molecular Characterization and Overexpression ofInternational journal of molecular sciences · 2018Article
- Transcriptome Analysis of JA Signal Transduction, Transcription Factors, and Monoterpene Biosynthesis Pathway in Response to Methyl Jasmonate Elicitation inInternational journal of molecular sciences · 2018Article
- Overexpression ofFrontiers in plant science · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gentiana macrophylla, a medicinal plant with significant pharmacological properties, contains the bioactive compound gentiopicroside. Methyl jasmonate (MeJA) is an effective elicitor for enhancing the production of such compounds. However, little is known about MeJA-mediated biosynthesis of gentiopicroside. We investigated this phenomenon as well as gene expression profiles to determine the molecular mechanisms for MeJA-mediated gentiopicroside biosynthesis and regulation in G. macrophylla. Our HPLC results showed that Gentiana macrophylla seedlings exposed to MeJA had significantly higher concentrations of gentiopicroside when compared with control plants. We used RNA sequencing to compare transcriptional profiles in seedlings treated for 5 d with either 0 μmol L-1 MeJA (C) or 250 μmol L-1 MeJA (M5) and detected differentially expressed genes (DEGs). In total, 77,482 unique sequences were obtained from approximately 34 million reads. Of these, 48,466 (57.46%) sequences were annotated based on BLASTs performed against public databases. We identified 5,206 DEGs between the C and M5 samples, including genes related to the α-lenolenic acid degradation pathway, JA signaling pathway, and gentiopicroside biosynthesis. Expression of numerous enzyme genes in the glycolysis pathway was significantly up-regulated. Many genes encoding transcription factors (e.g. ERF, bHLH, MYB, and WRKY) also responded to MeJA elicitation. Rapid acceleration of the glycolysis pathway that supplies precursors for IPP biosynthesis and up-regulates the expression of enzyme genes in that IPP pathway are probably most responsible for MeJA stimulation of gentiopicroside synthesis. Our qRT-PCR results showed that the expression profiles of 12 gentiopicroside biosynthesis genes were consistent with the RNA-Seq data. These results increase our understanding about how the gentiopicroside biosynthesis pathway in G. macrophylla responds to MeJA.
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