ArticleScientific reports2016
An automated approach to prepare tissue-derived spatially barcoded RNA-sequencing libraries.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Spatial transcriptome analysis of the tea tender shoot sheds light on transcriptional regulation of characteristic metabolites.Horticulture research · 2026Article
- Single-Cell Multi-Omics Profiling of Human Septal Myectomy Tissue: Toward Precision Medicine in Obstructive Hypertrophic Cardiomyopathy.Journal of personalized medicine · 2026Review
- Article
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- Advancing muscle aging and sarcopenia research through spatial transcriptomics.Osteoporosis and sarcopenia · 2025Review
- Automation of RNA-Seq Sample Preparation and Miniaturized Parallel Bioreactors Enable High-Throughput Differential Gene Expression Studies.Microorganisms · 2025Article
- Spatial Transcriptomics in Human Cardiac Tissue.International journal of molecular sciences · 2025Review
- Spatial transcriptomics reveals an unexpected impact of tau and tau pathology on the expression of transthyretin.Frontiers in aging neuroscience · 2025Article
- Fhl1, a new spatially specific protein, regulates vein graft neointimal hyperplasia.Clinical and translational medicine · 2024Article
- Molecular Lineages and Spatial Distributions of Subplate Neurons in the Human Fetal Cerebral Cortex.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Spatial transcriptomics reveals substantial heterogeneity in triple-negative breast cancer with potential clinical implications.Nature communications · 2024Article
- Host-pathogen interactions in the Plasmodium-infected mouse liver at spatial and single-cell resolution.Nature communications · 2024Article
- Spatial transcriptomics reveals gene interactions and signaling pathway dynamics in rat embryos with anorectal malformation.Cell biology and toxicology · 2024Article
- Towards high-throughput parallel imaging and single-cell transcriptomics of microbial eukaryotic plankton.PloS one · 2024Article
- Spatial transcriptomics in breast cancer: providing insight into tumor heterogeneity and promoting individualized therapy.Frontiers in immunology · 2024Review
- Dissecting mammalian reproduction with spatial transcriptomics.Human reproduction update · 2023Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing the nucleic acid content of individual cells or specific biological samples is becoming increasingly common. This drives the need for robust, scalable and automated library preparation protocols. Furthermore, an increased understanding of tissue heterogeneity has lead to the development of several unique sequencing protocols that aim to retain or infer spatial context. In this study, a protocol for retaining spatial information of transcripts has been adapted to run on a robotic workstation. The method spatial transcriptomics is evaluated in terms of robustness and variability through the preparation of reference RNA, as well as through preparation and sequencing of six replicate sections of a gingival tissue biopsy from a patient with periodontitis. The results are reduced technical variability between replicates and a higher throughput, processing four times more samples with less than a third of the hands on time, compared to the standard protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.