ArticleNature communications2016
Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 337 papers.
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Who cites it
337 citing papers in PubMed.
- CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.Plant, cell & environment · 2026Review
- Ecology and evolutionary dynamics of the A-strain maize streak virus and the decade-long strategies targeting streak-resistant maize.Virus research · 2026Review
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Transgene-free genome editing in plants.aBIOTECH · 2026Review
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- Genomics for next-generation wheat breeding.The plant genome · 2026Article
- Advances in Tissue Culture-Free Genetic Engineering and Genome Editing of Peanut.Molecular biotechnology · 2026Review
- Decoding plant physiology through systems biology: Integrative multi-omics and computational perspectives for next-generation crop design.Plant communications · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Genetic transformation and genome editing of flax (Frontiers in plant science · 2026Review
- Beyond GMOs: transgene-free gene-edited crops for global food security.Frontiers in plant science · 2026Review
- Breeding climate-resilient maize for food security in semi-arid sub-Saharan Africa: strategies and future directions.Frontiers in plant science · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Development ofFrontiers in genome editing · 2026Article
- Impact of wheat GRF4-GIF1 morphogenic regulators on transformation and genome editing efficiency in elite barley cultivars.Frontiers in plant science · 2026Article
- A voyage of reprogrammable metabolic bioengineering reshapes plant defense: from editing tools to synthetic systems.Frontiers in plant science · 2026Review
- Methylviologen resistance in loss-of-function mutants of the polyamine transporter gene OsLAT5.PloS one · 2026Article
- Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter.Scientific reports · 2025Article
277 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.
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