ArticleAMB Express2016
Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae.
Article in AMB Express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Enhancing myrcene biosynthesis in yeast through nuclear compartmentalization.Synthetic and systems biotechnology · 2027Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Efficient biosynthesis of the plant-derived diterpenoid 14-hydroxy-dehydroabietadiene in Saccharomyces cerevisiae.Microbial cell factories · 2026Article
- Implementation of the synthetic arabinose 5-phosphate dependent glycolaldehyde assimilation (SAGA) pathway inFrontiers in bioengineering and biotechnology · 2026Article
- Enhanced chlorogenic acid production from glucose via systematic metabolic engineering ofSynthetic and systems biotechnology · 2025Article
- Sucrose-driven carbon redox rebalancing eliminates the Crabtree effect and boosts energy metabolism in yeast.Nature communications · 2025Article
- Cell-Free Reaction System for ATP Regeneration from d-Fructose.ACS synthetic biology · 2025Article
- Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol inSynthetic and systems biotechnology · 2025Article
- Production of (R)-citramalate by engineeredMetabolic engineering communications · 2024Article
- Tuning Fatty Acid Profile and Yield inBioengineering (Basel, Switzerland) · 2023Article
- Engineering a Phosphoketolase Pathway to Supplement Cytosolic Acetyl-CoA inJournal of fungi (Basel, Switzerland) · 2023Article
- Increasing cellular fitness and product yields in Pseudomonas putida through an engineered phosphoketolase shunt.Microbial cell factories · 2023Article
- Article
- Engineering ofMetabolic engineering communications · 2022Review
- An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2022Article
- Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast.Nature chemical biology · 2022Article
- Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production.Synthetic and systems biotechnology · 2022Article
- Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2021Article
- Auxin-mediated induction of GAL promoters by conditional degradation of Mig1p improves sesquiterpene production in Saccharomyces cerevisiae with engineered acetyl-CoA synthesis.Microbial biotechnology · 2021Article
- Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain.Biotechnology for biofuels · 2021Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphoketolases catalyze an energy- and redox-independent cleavage of certain sugar phosphates. Hereby, the two-carbon (C2) compound acetyl-phosphate is formed, which enzymatically can be converted into acetyl-CoA-a key precursor in central carbon metabolism. Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. In this study, we aimed to compare and identify efficient heterologous phosphoketolase enzyme candidates that in yeast have the potential to reduce carbon loss compared to the native acetyl-CoA producing pathway by redirecting carbon flux directly from C5 and C6 sugars towards C2-synthesis. Nine phosphoketolase candidates were expressed in S. cerevisiae of which seven produced significant amounts of acetyl-phosphate after provision of sugar phosphate substrates in vitro. The candidates showed differing substrate specificities, and some demonstrated activity levels significantly exceeding those of candidates previously expressed in yeast. The conducted studies also revealed that S. cerevisiae contains endogenous enzymes capable of breaking down acetyl-phosphate, likely into acetate, and that removal of the phosphatases Gpp1 and Gpp2 could largely prevent this breakdown. An evaluation of in vivo function of a subset of phosphoketolases was conducted by monitoring acetate levels during growth, confirming that candidates showing high activity in vitro indeed showed increased acetate accumulation, but expression also decreased cellular fitness. The study shows that expression of several bacterial phosphoketolase candidates in S. cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds.
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