Evidence map›Paper›PMID 27848228›Full record

ArticleJournal of endocrinological investigation2017

High levels of DNA polymerase β mRNA corresponding with the high activity in Graves' thyroid tissue.

N Hayakawa, Y Sato, A Nagasaka, Y Mano, T Nagasaka, A Nakai, K Iwase, S Yoshida

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Journal of endocrinological investigation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 81% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

N HayakawaDepartment of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan.
Y SatoFaculty of Agriculture, Nagoya University, Nagoya, 466-8550, Japan.
A NagasakaDepartment of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan.
Y ManoFujita Health University, Toyoake, Aichi, 470-1192, Japan. manoyasu@fujita-hu.ac.jp.
T NagasakaDepartment of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, 113-8655, Japan.
A NakaiNakai Clinic, Chita, Aichi, 478-0041, Japan.
K IwaseDepartment of Surgery, School of Medicine, Fujita Health University, Toyoake, Aichi, 470-1192, Japan.
S YoshidaLaboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control , Nagoya University School of Medicne, Nagoya University, Nagoya, 466-8550, Japan.
Fujita Health University · JPNagoya University · JPThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionHigh DNA polymerase β activity has been observed in the thyroid tissue of patients with Graves' disease (Nagasaka et al. in Metabolism 37:1051-1054, 1988). This fact aroused our interest in whether the alteration of DNA polymerase β activity depends on DNA polymerase β (DNA poly β) mRNA levels, which may be modulated by thyroid-stimulating hormone (TSH) or thyroid-stimulating substances, i.e. TSH receptor antibody (TRAb).

resultAddition of TSH or TRAb to primary cultures of Graves' disease thyroid cells for 4 h led to no increase in DNA poly β mRNA levels. In contrast, thyroid hormone synthesizing enzyme, peroxidase, mRNA levels increased fivefold after coculture with TSH and TRAb, even though DNA poly β activity and mRNA levels are already significantly higher in Graves' disease thyroid tissues, compared with normal thyroid tissue. DISCUSSION: These results indicate that DNA poly β expression in Graves' disease thyroid cells may be maximally activated or plateau in response to thyroid-stimulating immunoglobulins, or that the activation of to poly β expression may occur via pathways other than the G protein and cyclic AMP system.

Indexed as

AutoantigensBlotting, NorthernCells, CulturedDNA Polymerase betaGraves DiseaseHumansImmunoglobulins, Thyroid-StimulatingIodide PeroxidaseIron-Binding ProteinsReceptors, ThyrotropinRNA, MessengerThyroid GlandThyroid HormonesThyrotropinAutoantigensDNA Polymerase betaImmunoglobulins, Thyroid-StimulatingIodide PeroxidaseIron-Binding ProteinsReceptors, ThyrotropinRNA, MessengerThyroid HormonesThyrotropinTPO protein, humanDNA polymeraseGraves’ diseaseIodide peroxidase and mRNAs

Identifiers

PMID27848228
OpenAlexW2557191560

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.