Evidence map›Paper›PMID 27848068›Full record

ArticleVirus genes2017

Inhibition of porcine transmissible gastroenteritis virus infection in porcine kidney cells using short hairpin RNAs targeting the membrane gene.

Li Wang, Xianjin Dai, Han Song, Peng Yuan, Zhou Yang, Wei Dong, Zhenhui Song

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Li WangDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Xianjin DaiDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Han SongDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Peng YuanDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Zhou YangDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Wei DongDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China.
Zhenhui SongDepartment of Veterinary Medicine, Southwest University, Rongchang Campus, Chongqing, 402460, People's Republic of China. szh7678@126.com.
Southwest University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The membrane (M) protein is the most abundant component of the porcine transmissible gastroenteritis virus (TGEV) particle. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, three plasmids (pRNAT-1, pRNAT-2, and pRNAT-3) expressing short hairpin RNAs were designed to target three different coding regions of the M gene of TGEV. The plasmids were constructed and transiently transfected into a porcine kidney cells, PK-15, to determine whether these constructs inhibited TGEV production. The analysis of cytopathic effects demonstrated that pRNAT-2 and pRNAT-3 could protect PK-15 cells against pathological changes specifically and efficiently. Additionally, indirect immunofluorescence and 50% tissue culture infectious dose (TCID

Indexed as

AnimalsGastroenteritis, Transmissible, of SwineKidneyMembrane ProteinsRNA InterferenceRNA, Small InterferingSwineTransmissible gastroenteritis virusVirus ReplicationMembrane ProteinsRNA, Small InterferingMembrane genePorcine kidney cellRNA interferenceShort hairpin RNATransmissible gastroenteritis virus

Identifiers

PMID27848068
PMCPMC7089173
OpenAlexW2549637171

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.