ArticleJournal of clinical microbiology2017
Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay.
Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- SERS-based spectral analysis of ITS1 PCR products for differentiation of three old world Leishmania species.Lasers in medical science · 2026Article
- TIM, a novel molecular toolbox for the detection and identification ofMicrobiology spectrum · 2026Article
- The diversity of sandflies (Psychodidae: Phlebotominae) and the presence of Leishmania spp. DNA in potential vectors of the Mbaracayú Forest Biosphere Reserve, Canindeyú, Paraguay: New records and findings.PLoS neglected tropical diseases · 2025Article
- Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran.Acta parasitologica · 2024Article
- Assessment of High-Resolution Melting Curve Analysis forPathogens (Basel, Switzerland) · 2024Article
- A Diagnostic Algorithm for Detection of Leishmania spp. in Human Fresh and Fixed Tissue Samples.The American journal of tropical medicine and hygiene · 2024Article
- Evaluation of Molecular Methods to Identify Chagas Disease and Leishmaniasis in Blood Donation Candidates in Two Brazilian Centers.Pathogens (Basel, Switzerland) · 2023Article
- Evaluation of the Diagnostic Sensitivity of the VIASURETransboundary and emerging diseases · 2023Article
- Applications of MALDI-TOF Mass Spectrometry to the Identification of Parasites and Arthropod Vectors of Human Diseases.Microorganisms · 2022Review
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
- Establish an allele-specific real-time PCR for Leishmania species identification.Infectious diseases of poverty · 2022Article
- Multiparametric analysis of host and parasite elements in new world tegumentary leishmaniasis.Frontiers in cellular and infection microbiology · 2022Article
- Cutaneous Leishmaniasis Caused by an Unknown Leishmania Strain, Arizona, USA.Emerging infectious diseases · 2021Article
- Identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods.Microorganisms · 2020Review
- An Atypical Case of Autochthonous Cutaneous Leishmaniasis Associated with Naturally Infected Phlebotomine Sand Flies in Texas, United States.The American journal of tropical medicine and hygiene · 2020Article
- Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis.PLoS neglected tropical diseases · 2020Article
- Duplex Real-Time PCR Assay for Clinical Differentiation ofThe American journal of tropical medicine and hygiene · 2020Article
- Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis.Parasites & vectors · 2020Article
- Semi-Quantitative, Duplexed qPCR Assay for the Detection ofTropical medicine and infectious disease · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp. Here we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green technology (LSG-qPCR), which uses genus-specific primers that target the ITS1 region and amplify DNA from at least 10 Leishmania spp., followed by analysis of the melting temperature (T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.