ArticleBMC genomics2016
Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- "Pathomorphogenic" Changes Caused by Citrus Bark Cracking Viroid and Transcription Factor TFIIIA-7ZF Variants Support Viroid Propagation in Tobacco.International journal of molecular sciences · 2023Article
- Comparative genomics of flowering behavior inFrontiers in plant science · 2023Article
- MicroRNAs in Medicinal Plants.International journal of molecular sciences · 2022Review
- Identification and Characterization ofPlants (Basel, Switzerland) · 2021Article
- Next-Generation Sequencing Identification and Characterization of MicroRNAs in Dwarfed Citrus Trees Infected With Citrus Dwarfing Viroid in High-Density Plantings.Frontiers in microbiology · 2021Article
- Progress in 50 years of viroid research-Molecular structure, pathogenicity, and host adaptation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Article
- Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes.International journal of molecular sciences · 2020Article
- Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants.International journal of molecular sciences · 2020Article
- Evaluation of Disease Severity and Global Transcriptome Response Induced byInternational journal of molecular sciences · 2019Article
- Revisiting the Role of Transcription Factors in Coordinating the Defense Response AgainstViruses · 2019Article
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- Differential Expression of MicroRNAs During Root Formation inOpen life sciences · 2019Article
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- Plant Small Non-coding RNAs and Their Roles in Biotic Stresses.Frontiers in plant science · 2018Review
- Transcriptome sequencing reveals novel Citrus bark cracking viroid (CBCVd) variants from citrus and their molecular characterization.PloS one · 2018Article
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHop (Humulus lupulus L.) plants are grown primarily for the brewing industry and have been used as a traditional medicinal herb for a long time. Severe hop stunt disease caused by the recently discovered Citrus bark cracking viroid (CBCVd) is one of the most devastating diseases among other viroid infections in hop. MicroRNAs (miRNAs) are a class of non-coding small RNAs that play important roles in gene expression regulation. To identify miRNAs in hop and their response to CBCVd-infection, two small RNA (sRNA) libraries were prepared from healthy and CBCVd-infected hop plants and were investigated by high throughput sequencing.
resultsA total of 67 conserved and 49 novel miRNAs were identified. Among them, 36 conserved and 37 novel miRNAs were found to be differentially recovered in response to CBCVd-infection. A total of 311 potential targets was predicted for conserved and novel miRNAs based on a sequence homology search using hop transcriptome data. The majority of predicted targets significantly belonged to transcriptional factors that may regulate hop leaf, root and cone growth and development. In addition, the identified miRNAs might also play an important roles in other cellular and metabolic processes, such as signal transduction, stress response and other physiological processes, including prenylflavonoid biosynthesis pathways. Quantitative real time PCR analysis of selected targets revealed their negative correlation with their corresponding CBCVd-responsive miRNAs.
conclusionsBased on the results, we concluded that CBCVd-responsive miRNAs modulate several hormone pathways and transcriptional factors that play important roles in the regulation of metabolism, growth and development. These results provide a framework for further analysis of regulatory roles of sRNAs in plant defense mechanism including other hop infecting viroids in particular.
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