ArticleInternational journal of molecular sciences2016
Generation and Characterisation of a Reference Transcriptome for Lentil (Lens culinaris Medik.).
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Article
- Transcriptomic insights into shared responses to Fusarium crown rot infection and drought stresses in bread wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- The Prospects of gene introgression from crop wild relatives into cultivated lentil for climate change mitigation.Frontiers in plant science · 2023Review
- Omics Path to Increasing Productivity in Less-Studied Crops Under Changing Climate-Lentil a Case Study.Frontiers in plant science · 2022Review
- Advances in lentil production through heterosis: Evaluating generations and breeding systems.PloS one · 2022Article
- Genomics Associated Interventions for Heat Stress Tolerance in Cool Season Adapted Grain Legumes.International journal of molecular sciences · 2021Review
- Article
- The Potential of Selected Agri-Food Loss and Waste to Contribute to a Circular Economy: Applications in the Food, Cosmetic and Pharmaceutical Industries.Molecules (Basel, Switzerland) · 2021Review
- Article
- Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil.Frontiers in plant science · 2021Article
- Prospects of next generation sequencing in lentil breeding.Molecular biology reports · 2020Review
- Pulse Crop Genetics for a Sustainable Future: Where We Are Now and Where We Should Be Heading.Frontiers in plant science · 2020Review
- Characterization of Genetic and Allelic Diversity Amongst Cultivated and Wild Lentil Accessions for Germplasm Enhancement.Frontiers in genetics · 2020Article
- Generation of a Comprehensive Transcriptome Atlas and Transcriptome Dynamics in Medicinal Cannabis.Scientific reports · 2019Article
- Using a transcriptome sequencing approach to explore candidate resistance genes against stemphylium blight in the wild lentil species Lens ervoides.BMC plant biology · 2019Article
- Genome wide transcriptome analysis reveals vital role of heat responsive genes in regulatory mechanisms of lentil (Lens culinaris Medikus).Scientific reports · 2019Article
- Comparative RNA-seq analysis of the drought-sensitive lentil (Lens culinaris) root and leaf under short- and long-term water deficits.Functional & integrative genomics · 2019Article
- Towards Exploitation of Adaptive Traits for Climate-Resilient Smart Pulses.International journal of molecular sciences · 2019Review
- Validation of reference genes for qRT-PCR data normalisation in lentil (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-Seq using second-generation sequencing technologies permits generation of a reference unigene set for a given species, in the absence of a well-annotated genome sequence, supporting functional genomics studies, gene characterisation and detailed expression analysis for specific morphophysiological or environmental stress response traits. A reference unigene set for lentil has been developed, consisting of 58,986 contigs and scaffolds with an N50 length of 1719 bp. Comparison to gene complements from related species, reference protein databases, previously published lentil transcriptomes and a draft genome sequence validated the current dataset in terms of degree of completeness and utility. A large proportion (98%) of unigenes were expressed in more than one tissue, at varying levels. Candidate genes associated with mechanisms of tolerance to both boron toxicity and time of flowering were identified, which can eventually be used for the development of gene-based markers. This study has provided a comprehensive, assembled and annotated reference gene set for lentil that can be used for multiple applications, permitting identification of genes for pathway-specific expression analysis, genetic modification approaches, development of resources for genotypic analysis, and assistance in the annotation of a future lentil genome sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.