ReviewMolecules (Basel, Switzerland)2016
Ligand Fishing: A Remarkable Strategy for Discovering Bioactive Compounds from Complex Mixture of Natural Products.
Review in Molecules (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Magnetic Bead-Based Ligand Fishing Identifies SARS-CoV-2 Main Protease Inhibitors From Reynoutria japonica.Chemistry & biodiversity · 2026Article
- Functional processing enhances hepatic targeting: the OAT2/MRP2 mechanism of vinegar-processed Cyperi Rhizoma.Frontiers in nutrition · 2026Article
- Optimizing blood-brain barrier permeability: the effect of wine processing on alkaloids in Phellodendri Chinensis Cortex.Journal of natural medicines · 2025Article
- Screening of monoamine oxidase B inhibitors in Tibetan strawberry by ligand fishing based on enzyme functionalized cellulose filter paper.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2024Article
- Article
- High throughput identification of human monoclonal antibodies and heavy-chain-only antibodies to treat snakebite.Toxicon: X · 2024Article
- Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury.Frontiers in pharmacology · 2024Article
- Aggregation of magnetic nanoparticles functionalized with trans-resveratrol in aqueous solution.Discover nano · 2023Article
- A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing.Journal of pharmaceutical analysis · 2022Article
- Rapid Screening of Lipase Inhibitors inMolecules (Basel, Switzerland) · 2022Article
- Natural product drug discovery in the artificial intelligence era.Chemical science · 2022Review
- Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits ofFrontiers in nutrition · 2022Article
- Recent advance in the discovery of tyrosinase inhibitors from natural sources via separation methods.Journal of enzyme inhibition and medicinal chemistry · 2021Review
- Considerations to Be Taken When Carrying Out Medicinal Plant Research-What We Learn from an Insight into the ICPharmaceuticals (Basel, Switzerland) · 2021Review
- An Integrated Strategy for the Detection, Dereplication, and Identification of DNA-Binding Biomolecules from Complex Natural Product Mixtures.Journal of natural products · 2021Article
- Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract.PloS one · 2021Article
- Immobilization of cell membrane onto a glucose-Zn-based porous coordination polymer and its application to rapid screening of potentially active compounds from Vaccinium corymbosum L. leaves.Mikrochimica acta · 2020Article
- Recent advances in screening active components from natural products based on bioaffinity techniques.Acta pharmaceutica Sinica. B · 2020Review
- Opportunities and Challenges for Biosensors and Nanoscale Analytical Tools for Pandemics: COVID-19.ACS nano · 2020Review
- Natural Products as Modulators of Sirtuins.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of active compounds from natural products is a critical and challenging task in drug discovery pipelines. Besides commonly used bio-guided screening approaches, affinity selection strategy coupled with liquid chromatography or mass spectrometry, known as ligand fishing, has been gaining increasing interest from researchers. In this review, we summarized this emerging strategy and categorized those methods as off-line or on-line mode according to their features. The separation principles of ligand fishing were introduced based on distinct analytical techniques, including biochromatography, capillary electrophoresis, ultrafiltration, equilibrium dialysis, microdialysis, and magnetic beads. The applications of ligand fishing approaches in the discovery of lead compounds were reviewed. Most of ligand fishing methods display specificity, high efficiency, and require less sample pretreatment, which makes them especially suitable for screening active compounds from complex mixtures of natural products. We also summarized the applications of ligand fishing in the modernization of Traditional Chinese Medicine (TCM), and propose some perspectives of this remarkable technique.
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