Evidence map›Paper›PMID 27845444›Full record

ArticleScientific reports2016

Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown.

MaFeng Liu, MengYi Wang, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, KunFeng Sun, Qiao Yang, Ying Wu, XiaoYue Chen and 2 more

Abstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

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  5. Functional characterization of a TerC family protein ofApplied and environmental microbiology · 2024
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  12. Natural Transformation ofFrontiers in microbiology · 2021
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  13. PutativeFrontiers in microbiology · 2021
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  18. DprA Is Essential for Natural Competence inFrontiers in genetics · 2019
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

MaFeng LiuInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
MengYi WangInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
DeKang ZhuAvian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
MingShu WangInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
RenYong JiaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
Shun ChenInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
KunFeng SunInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
Qiao YangInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
Ying WuInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
XiaoYue ChenInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
Francis BivilleUnité des Infections Bactériennes Invasives, Département Infection et Epidémiologie, Institut Pasteur, Paris, France.
AnChun ChengInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Riemerella anatipestifer is a duck pathogen that has caused serious economic losses to the duck industry worldwide. Despite this, there are few reported studies of the physiological and pathogenic mechanisms of Riemerella anatipestifer infection. In previous study, we have shown that TonB1 and TonB2 were involved in hemin uptake. TonB family protein (TbfA) was not investigated, since knockout of this gene was not successful at that time. Here, we used a plasmid based gene over-expression and knockdown to investigate its function. First, we constructed three Escherichia-Riemerella anatipestifer shuttle vectors containing three different native Riemerella anatipestifer promoters. The shuttle plasmids were introduced into Riemerella anatipestifer ATCC11845 by conjugation at an efficiency of 5 × 10

Indexed as

Bacterial ProteinsCarrier ProteinsRiemerellaEscherichia coliGene Knockdown TechniquesHeminPlasmidsBacterial ProteinsCarrier ProteinsHemin

Identifiers

PMID27845444
PMCPMC5109031

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.