ArticleScientific reports2016
Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Polarized hyperspectral and polarized light microscopic imaging for enhanced visualization of white blood cells.Journal of biomedical optics · 2026Article
- Towards noninvasive white blood cell count.Biomedical optics express · 2025Article
- Deciphering neutrophil dynamics: Enhanced phagocytosis of elastic particles and impact on vascular-targeted carrier performance.Science advances · 2025Article
- In Vivo Visualization of Intravascular Patrolling Immune Cells in the Primate Eye.Investigative ophthalmology & visual science · 2024Article
- Predicting DNA damage response in non-small cell lung cancer organoids via simultaneous label-free autofluorescence multiharmonic microscopy.Redox biology · 2024Article
- Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation.Scientific reports · 2023Article
- Leukocyte differentiation in bronchoalveolar lavage fluids using higher harmonic generation microscopy and deep learning.PloS one · 2023Article
- Boundary-Preserved Deep Denoising of Stochastic Resonance Enhanced Multiphoton Images.IEEE journal of translational engineering in health and medicine · 2022Article
- Laser tweezers as a biophotonic tool to investigate the efficacy of living sickle red blood cells in response to optical deformation.Biophysical reviews · 2021Article
- Labeling and tracking of immune cells in ex vivo human skin.Nature protocols · 2021Article
- Label-free assessment of hemodynamics in individual cortical brain vessels using third harmonic generation microscopy.Biomedical optics express · 2020Article
- Report on fluorescence lifetime imaging using multiphoton laser scanning microscopy targeting sentinel lymph node diagnostics.Journal of biomedical optics · 2020Article
- Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- Label-free characterization of white blood cells using fluorescence lifetime imaging and flow-cytometry: molecular heterogeneity and erythrophagocytosis [Invited].Biomedical optics express · 2019Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
- Biophysical investigation of living monocytes in flow by collaborative coherent imaging techniques.Biomedical optics express · 2018Article
- Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging.Scientific reports · 2018Article
- Non-invasive detection of severe neutropenia in chemotherapy patients by optical imaging of nailfold microcirculation.Scientific reports · 2018Article
- In vivo noninvasive microscopy of human leucocytes.Scientific reports · 2017Article
- Single-cell screening of multiple biophysical properties in leukemia diagnosis from peripheral blood by pure light scattering.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count the number of each of the three leukocyte types both in vitro and in vivo. The THG imaging-based counting results agreed well with conventional blood count results. In the future, we believe that the combination of this THG microscopy-based imaging cytometry approach with advanced texture analysis of sub-cellular features can differentiate and count more types of blood cells with smaller quantities of blood.
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