ArticleScientific reports2016
The RNA-binding protein CsrA plays a central role in positively regulating virulence factors in Erwinia amylovora.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Virulence Regulation and Lifestyle Transitions: The Role of c-di-GMP and Two-Component Systems in Erwinia amylovora and Their Evolutionary Context Within Enterobacterales.Molecular plant pathology · 2026Review
- Pseudomonads coordinate innate defense against viruses and bacteria with a single regulatory system.Cell host & microbe · 2025Article
- Integrated Transcriptomic and Proteomic Analyses Reveal CsrA-Mediated Regulation of Virulence and Metabolism inMicroorganisms · 2025Article
- Pseudomonads coordinate innate defense against viruses and bacteria with a single regulatory system.bioRxiv : the preprint server for biology · 2025Article
- Article
- Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection.Microbiology spectrum · 2023Article
- Phage Cocktail in Combination with Kasugamycin as a Potential Treatment for Fire Blight Caused byAntibiotics (Basel, Switzerland) · 2022Article
- CsrD regulates amylovoran biosynthesis and virulence in Erwinia amylovora in a novel cyclic-di-GMP dependent manner.Molecular plant pathology · 2022Article
- Identification of novel virulence factors in Erwinia amylovora through temporal transcriptomic analysis of infected apple flowers under field conditions.Molecular plant pathology · 2022Article
- The RsmA RNA-Binding Proteins inFrontiers in plant science · 2021Article
- Comparative Genomics and Evolutionary Analysis of RNA-Binding Proteins of the CsrA Family in the GenusFrontiers in molecular biosciences · 2020Article
- Homologues of the RNA binding protein RsmA in Pseudomonas syringae pv. tomato DC3000 exhibit distinct binding affinities with non-coding small RNAs and have distinct roles in virulence.Molecular plant pathology · 2019Article
- Article
- Lon protease modulates virulence traits in Erwinia amylovora by direct monitoring of major regulators and indirectly through the Rcs and Gac-Csr regulatory systems.Molecular plant pathology · 2018Article
- Integration of multiple stimuli-sensing systems to regulate HrpS and type III secretion system in Erwinia amylovora.Molecular genetics and genomics : MGG · 2018Article
- Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes.Archives of microbiology · 2017Article
- Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars - susceptible and resistant to fire blight.BMC genomics · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The GacS/GacA two-component system (also called GrrS/GrrA) is a global regulatory system which is highly conserved among gamma-proteobacteria. This system positively regulates non-coding small regulatory RNA csrB, which in turn binds to the RNA-binding protein CsrA. However, how GacS/GacA-Csr system regulates virulence traits in E. amylovora remains unknown. Results from mutant characterization showed that the csrB mutant was hypermotile, produced higher amount of exopolysaccharide amylovoran, and had increased expression of type III secretion (T3SS) genes in vitro. In contrast, the csrA mutant exhibited complete opposite phenotypes, including non-motile, reduced amylovoran production and expression of T3SS genes. Furthermore, the csrA mutant did not induce hypersensitive response on tobacco or cause disease on immature pear fruits, indicating that CsrA is a positive regulator of virulence factors. These findings demonstrated that CsrA plays a critical role in E. amylovora virulence and suggested that negative regulation of virulence by GacS/GacA acts through csrB sRNA, which binds to CsrA and neutralizes its positive effect on T3SS gene expression, flagellar formation and amylovoran production. Future research will be focused on determining the molecular mechanism underlying the positive regulation of virulence traits by CsrA.
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