Evidence map›Paper›PMID 27844437›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution.

Anirban Chakraborty, Cong A Meng, Steven M Block

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
5.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Approaches for measuring the dynamics of RNA-protein interactions.Wiley interdisciplinary reviews. RNA · 2020
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Anirban ChakrabortyDepartment of Biology, Stanford University, Stanford, CA, USA.
Cong A MengDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Steven M BlockDepartment of Biology and Department of Applied Physics, Stanford University, Stanford, CA, USA. sblock@stanford.edu.
Stanford University · US

Funding

Nucleic Acid Enzymes and Nucleic Acids Studied at the Molecular LevelR37GM057035 · NIGMS · STANFORD UNIVERSITY · PI BLOCK, STEVEN M · 2010 to 2019
$5.7M
NIGMS NIH HHS R37 GM057035
6 · The paper itself

Abstract

During transcriptional elongation, RNA polymerases (RNAP) employ a stepping mechanism to translocate along the DNA template while synthesizing RNA. Optical trapping assays permit the progress of single molecules of RNA polymerase to be monitored in real time, at resolutions down to the level of individual base pairs. Additionally, optical trapping assays permit the application of exquisitely controlled, external forces on RNAP. Responses to such forces can reveal details of the load-dependent kinetics of transcriptional elongation and pausing. Traditionally, the bacterial form of RNAP from E. coli has served as a model for the study of transcriptional elongation using optical traps. However, it is now feasible to perform optical trapping experiments using the eukaryotic polymerase, RNAPII, as well. In this report, we describe the methods to perform optical trapping transcriptional elongation assays with both prokaryotic RNAP and eukaryotic RNAPII. We provide detailed instructions on how to reconstitute transcription elongation complexes, derivatize beads used in the assays, and perform optical trapping measurements.

Indexed as

Optical TweezersBase PairingDNA-Directed RNA PolymerasesEscherichia coliMicroscopyRNASingle Molecule ImagingSpectrum AnalysisTranscriptional Elongation FactorsTranscription, GeneticDNA-Directed RNA PolymerasesRNATranscriptional Elongation FactorsOptical trappingRNA polymeraseRNA polymerase IISingle-moleculeTranscription

Identifiers

PMID27844437
PMCPMC10167922
OpenAlexW2550787419

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.