A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization.

Stephen R Okoniewski, Ashley R Carter, Thomas T Perkins

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Stephen R OkoniewskiJILA, National Institute of Standards and Technology, and University of Colorado, Boulder, CO, 80309, USA.

Ashley R CarterDepartment of Physics and Astronomy, Amherst College, Amherst, MA, 01002, USA.

Thomas T PerkinsJILA, National Institute of Standards and Technology, and University of Colorado, Boulder, CO, 80309, USA. tperkins@jila.colorado.edu.

Funding

Predoctoral Training Molecular BiophysicsT32GM065103 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 2002 to 2021

$4.3M

NIGMS NIH HHS T32 GM065103

6 · The paper itself

Abstract

Optical traps can measure bead motions with Å-scale precision. However, using this level of precision to infer 1-bp motion of molecular motors along DNA is difficult, since a variety of noise sources degrade instrumental stability. In this chapter, we detail how to improve instrumental stability by (1) minimizing laser pointing, mode, polarization, and intensity noise using an acousto-optical-modulator mediated feedback loop and (2) minimizing sample motion relative to the optical trap using a three-axis piezo-electric-stage mediated feedback loop. These active techniques play a critical role in achieving a surface stability of 1 Å in 3D over tens of seconds and a 1-bp stability and precision in a surface-coupled optical trap over a broad bandwidth (Δf = 0.03-2 Hz) at low force (6 pN). These active stabilization techniques can also aid other biophysical assays that would benefit from improved laser stability and/or Å-scale sample stability, such as atomic force microscopy and super-resolution imaging.

Indexed as

Optical TweezersOptics and PhotonicsLasersMicroscopyOptical FibersActive stabilizationForce spectroscopyOptical trapOptical tweezersSingle molecule

Identifiers

PMID27844426

DOI10.1007/978-1-4939-6421-5_4

PMCPMC5510953

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.