Evidence map›Paper›PMID 27844275›Full record

ArticleThe protein journal2016

Energetics of Glutathione Binding to Human Eukaryotic Elongation Factor 1 Gamma: Isothermal Titration Calorimetry and Molecular Dynamics Studies.

Thabiso N Tshabalala, Mihai-Silviu Tomescu, Allan Prior, Vijayakumar Balakrishnan, Yasien Sayed, Heini W Dirr, Ikechukwu Achilonu

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Article in The protein journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Thabiso N TshabalalaProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Mihai-Silviu TomescuProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Allan PriorSchool of Chemistry, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Vijayakumar BalakrishnanProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Yasien SayedProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Heini W DirrProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa.
Ikechukwu AchilonuProtein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg, 2050, South Africa. Ikechukwu.Achilonu@wits.ac.za.ORCID http://orcid.org/0000-0002-8658-956X
University of the Witwatersrand · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The energetics of ligand binding to human eukaryotic elongation factor 1 gamma (heEF1γ) was investigated using reduced glutathione (GSH), oxidised glutathione (GSSG), glutathione sulfonate and S-hexylglutathione as ligands. The experiments were conducted using isothermal titration calorimetry, and the findings were supported using computational studies. The data show that the binding of these ligands to heEF1γ is enthalpically favourable and entropically driven (except for the binding of GSSG). The full length heEF1γ binds GSSG with lower affinity (K

Indexed as

CalorimetryGene ExpressionGlutathioneGlutathione DisulfideGlutathione TransferaseHumansHydrogen BondingKineticsLigandsMolecular Dynamics SimulationPeptide Elongation Factor 1Protein BindingProtein Structure, SecondaryRecombinant ProteinsStructural Homology, ProteinTemperatureeEF1B-gamma protein, humanGlutathioneGlutathione Disulfideglutathione sulfonateGlutathione TransferasehexylglutathioneLigandsPeptide Elongation Factor 1Recombinant ProteinsCHARMm-dockingGlutathione-binding energeticsHomology modellingHuman eukaryotic elongation factor 1 gammaIsothermal titration calorimetryMolecular dynamics simulations

Identifiers

PMID27844275
OpenAlexW2555188870

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.