Evidence map›Paper›PMID 27843954›Full record

ArticleJournal of diabetes research2016

Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability.

Bradley P Weegman, Venkata Sunil Kumar Sajja, Thomas M Suszynski, Michael D Rizzari, William E Scott Iii, Jennifer P Kitzmann, Kate R Mueller, Thomas R Hanley, David J Kennedy, Paul W Todd and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Bradley P WeegmanCMRR, Department of Radiology, University of Minnesota, Minneapolis, MN, USA; Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0002-2148-8979
Venkata Sunil Kumar SajjaDepartment of Chemical Engineering, Auburn University, Auburn, AL, USA.
Thomas M SuszynskiSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.ORCID 0000-0002-3515-8807
Michael D RizzariSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.
William E Scott IiiSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA; Department of Surgery, University of Arizona, Tucson, AZ, USA.
Jennifer P KitzmannDepartment of Surgery, University of Arizona, Tucson, AZ, USA.
Kate R MuellerSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA; Department of Surgery, University of Arizona, Tucson, AZ, USA.ORCID 0000-0002-8638-365X
Thomas R HanleyDepartment of Chemical Engineering, Auburn University, Auburn, AL, USA.ORCID 0000-0003-4465-202X
David J KennedyMobileMedTek Inc., Louisville, KY 40206, USA.
Paul W ToddTechshot, Inc., Greenville, IN, USA.
Appakalai N BalamuruganClinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KY 40202, USA.
Bernhard J HeringSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.
Klearchos K PapasSchulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA; Department of Surgery, University of Arizona, Tucson, AZ, USA.ORCID 0000-0002-4554-0911

Funding

Magnetic Flow Sorter for Pancreatic Islet IsolationR44DK072647 · NIDDK · TECHSHOT, INC. · PI BOLAND, EUGENE DAVID · 2008 to 2015
$3.8M
Magnetic Separation of Liberated Islets During IsolationR01DK068717 · NIDDK · UNIVERSITY OF MINNESOTA TWIN CITIES · PI PAPAS, KLEARCHOS K · 2004 to 2005
$371k
NIDDK NIH HHS R01 DK068717NIDDK NIH HHS R44 DK072647
6 · The paper itself

Abstract

Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield. In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions. Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications. Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process. Unlabeled islets from the CDL were purified by conventional DG. Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA,

Indexed as

Islets of LangerhansIslets of Langerhans TransplantationMagnetsAnimalsCentrifugation, Density GradientSwine

Identifiers

PMID27843954
PMCPMC5097811

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.