ArticleJournal of visualized experiments : JoVE2016
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Optimizing CRISPR/Cas9 mutagenesis in Drosophila dendritic arborization neurons to avoid cytotoxicity.G3 (Bethesda, Md.) · 2026Article
- Drosophila FMRP controls miR-276-mediated regulation of nejire mRNA for space-filling dendrite development.G3 (Bethesda, Md.) · 2022Article
- Decreasing pdzd8-mediated mito-ER contacts improves organismal fitness and mitigates AβLife science alliance · 2022Article
- The ELAV/Hu protein Found in neurons regulates cytoskeletal and ECM adhesion inputs for space-filling dendrite growth.PLoS genetics · 2020Article
- A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.The EMBO journal · 2020Article
- Molecular to organismal chirality is induced by the conserved myosin 1D.Science (New York, N.Y.) · 2018Article
- β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Enclosure of Dendrites by Epidermal Cells Restricts Branching and Permits Coordinated Development of Spatially Overlapping Sensory Neurons.Cell reports · 2017Article
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Authors and funding
2 authors.
Funding
Abstract
Drosophila larval dendritic arborization (da) neurons are a popular model for investigating mechanisms of neuronal morphogenesis. Da neurons develop in communication with the epidermal cells they innervate and thus their analysis benefits from in situ visualization of both neuronally and epidermally expressed proteins by immunofluorescence. Traditional methods of preparing larval fillets for immunofluorescence experiments leave intact the muscle tissue that covers most of the body wall, presenting several challenges to imaging neuronal and epidermal proteins. Here we describe a method for removing muscle tissue from Drosophila larval fillets. This protocol enables imaging of proteins that are otherwise obscured by muscle tissue, improves signal to noise ratio, and facilitates the use of super-resolution microscopy to study da neuron development.
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