ArticleJournal of visualized experiments : JoVE2016
Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- An interaction between HP1 and the Chromosomal Passenger Complex Initiates Acentrosomal Spindle Assembly inbioRxiv : the preprint server for biology · 2026Article
- Distinct checkpoint and homolog biorientation pathways regulate meiosis I in Drosophila oocytes.PLoS genetics · 2025Article
- Distinct checkpoint and homolog biorientation pathways regulate meiosis I inbioRxiv : the preprint server for biology · 2024Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inMolecular biology of the cell · 2024Article
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.PLoS genetics · 2023Article
- B chromosomes reveal a female meiotic drive suppression system in Drosophila melanogaster.Current biology : CB · 2023Article
- Multiple pools of PP2A regulate spindle assembly, kinetochore attachments and cohesion in Drosophila oocytes.Journal of cell science · 2021Article
- Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules.The Journal of cell biology · 2021Article
- Importin-9 regulates chromosome segregation and packaging in Drosophila germ cells.Journal of cell science · 2021Article
- Regulation of Trpm activation and calcium wave initiation during Drosophila egg activation.Molecular reproduction and development · 2020Article
- Sister centromere fusion during meiosis I depends on maintaining cohesins and destabilizing microtubule attachments.PLoS genetics · 2019Article
- Maternal Proteins That Are Phosphoregulated upon Egg Activation Include Crucial Factors for Oogenesis, Egg Activation and Embryogenesis inG3 (Bethesda, Md.) · 2018Article
- Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes.Journal of visualized experiments : JoVE · 2017Article
- Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes.Genetics · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Chromosome segregation in human oocytes is error prone, resulting in aneuploidy, which is the leading genetic cause of miscarriage and birth defects. The study of chromosome behavior in oocytes from model organisms holds much promise to uncover the molecular basis of the susceptibility of human oocytes to aneuploidy. Drosophila melanogaster is amenable to genetic manipulation, with over 100 years of research, community, and technique development. Visualizing chromosome behavior and spindle assembly in Drosophila oocytes has particular challenges, however, due primarily to the presence of membranes surrounding the oocyte that are impenetrable to antibodies. We describe here protocols for the collection, preparation, and imaging of meiosis I spindle assembly and chromosome behavior in Drosophila oocytes, which allow the molecular dissection of chromosome segregation in this important model organism.
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