ArticleJournal of visualized experiments : JoVE2016
The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Coupling intraoperative bioprinting and surgical micropuncture for synergistic scaffold vascularization.Biomaterials · 2026Article
- Mesenchymal stem cell therapy for breast cancer-related secondary lymphedema (Review).Molecular and clinical oncology · 2025Review
- In Vivo Vascularization of Cell-Supplemented Spider Silk-Based Hydrogels in the Arteriovenous Loop Model.Biomimetics (Basel, Switzerland) · 2025Article
- Biofabrication of 3D-printed, pre-cross-linked alginate dialdehyde-gelatin (ADA-GEL) scaffolds for anFrontiers in bioengineering and biotechnology · 2025Article
- A vascularizedMaterials today. Bio · 2024Article
- Perspectives on the Current State of Bioprinted Skin Substitutes for Wound Healing.Biomedicines · 2023Review
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- Article
- Review
- An Innovative Arteriovenous (AV) Loop Breast Cancer Model Tailored for Cancer Research.Bioengineering (Basel, Switzerland) · 2022Article
- Microsurgical Transplantation of Pedicled Muscles in an Isolation Chamber-A Novel Approach to Engineering Muscle Constructs via Perfusion-Decellularization.Journal of personalized medicine · 2022Article
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
- Unlocking mammalian regeneration through hypoxia inducible factor one alpha signaling.Biomaterials · 2021Review
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Combining Vascularization Strategies in Tissue Engineering: The Faster Road to Success?Frontiers in bioengineering and biotechnology · 2020Article
- Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization.Journal of tissue engineering and regenerative medicine · 2019Review
- Material-Induced Venosome-Supported Bone Tubes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Reconstruction of composite defects of the scalp and neurocranium-a treatment algorithm from local flaps to combined AV loop free flap reconstruction.World journal of surgical oncology · 2018Article
- Mesenchymal stem cells promote lymphangiogenic properties of lymphatic endothelial cells.Journal of cellular and molecular medicine · 2018Article
- Evaluation of a Miniaturized Biologically Vascularized Scaffold in vitro and in vivo.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A functional blood vessel network is a prerequisite for the survival and growth of almost all tissues and organs in the human body. Moreover, in pathological situations such as cancer, vascularization plays a leading role in disease progression. Consequently, there is a strong need for a standardized and well-characterized in vivo model in order to elucidate the mechanisms of neovascularization and develop different vascularization approaches for tissue engineering and regenerative medicine. We describe a microsurgical approach for a small animal model for induction of a vascular axis consisting of a vein and artery that are anastomosed to an arteriovenous (AV) loop. The AV loop is transferred to an enclosed implantation chamber to create an isolated microenvironment in vivo, which is connected to the living organism only by means of the vascular axis. Using 3D imaging (MRI, micro-CT) and immunohistology, the growing vasculature can be visualized over time. By implanting different cells, growth factors and matrices, their function in blood vessel network formation can be analyzed without any disturbing influences from the surroundings in a well controllable environment. In addition to angiogenesis and antiangiogenesis studies, the AV loop model is also perfectly suited for engineering vascularized tissues. After a certain prevascularization time, the generated tissues can be transplanted into the defect site and microsurgically connected to the local vessels, thereby ensuring immediate blood supply and integration of the engineered tissue. By varying the matrices, cells, growth factors and chamber architecture, it is possible to generate various tissues, which can then be tailored to the individual patient's needs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.