ArticleJournal of visualized experiments : JoVE2016
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Synthetic microbial communities for studying the fate and ecological role of specialized metabolites.Essays in biochemistry · 2026Review
- Swarming Motility Assays in Salmonella.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Complex extracellular biology drives surface competition during colony expansion in Bacillus subtilis.The ISME journal · 2022Article
- Kin Discrimination Modifies Strain Distribution, Spatial Segregation, and Incorporation of Extracellular Matrix Polysaccharide Mutants of Bacillus subtilis Strains into Mixed Floating Biofilms.Applied and environmental microbiology · 2022Article
- Article
- Adaptation and phenotypic diversification of Bacillus thuringiensis biofilm are accompanied by fuzzy spreader morphotypes.NPJ biofilms and microbiomes · 2022Article
- Surveying a Swarm: Experimental Techniques To Establish and Examine Bacterial Collective Motion.Applied and environmental microbiology · 2022Article
- Deletion of Rap-Phr systems in Bacillus subtilis influences in vitro biofilm formation and plant root colonization.MicrobiologyOpen · 2021Article
- Article
- Fungal hyphae colonization byBiofilm · 2019Article
- Chance and pleiotropy dominate genetic diversity in complex bacterial environments.Nature microbiology · 2019Article
- Division of Labor during Biofilm Matrix Production.Current biology : CB · 2018Article
- Chromosomal barcoding as a tool for multiplexed phenotypic characterization of laboratory evolved lineages.Scientific reports · 2018Article
- Presence of Calcium Lowers the Expansion of Bacillus subtilis Colony Biofilms.Microorganisms · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbes provide an intriguing system to study social interaction among individuals within a population. The short generation times and relatively simple genetic modification procedures of microbes facilitate the development of the sociomicrobiology field. To assess the fitness of certain microbial species, selected strains or their genetically modified derivatives within one population, can be fluorescently labelled and tracked using microscopy adapted with appropriate fluorescence filters. Expanding colonies of diverse microbial species on agar media can be used to monitor the spatial distribution of cells producing distinctive fluorescent proteins. Here, we present a detailed protocol for the use of green- and red-fluorescent protein producing bacterial strains to follow spatial arrangement during surface colonization, including flagellum-driven community movement (swarming), exopolysaccharide- and hydrophobin-dependent growth mediated spreading (sliding), and complex colony biofilm formation. Non-domesticated isolates of the Gram-positive bacterium, Bacillus subtilis can be utilized to scrutinize certain surface spreading traits and their effect on two-dimensional distribution on the agar-solidified medium. By altering the number of cells used to initiate colony biofilms, the assortment levels can be varied on a continuous scale. Time-lapse fluorescent microscopy can be used to witness the interaction between different phenotypes and genotypes at a certain assortment level and to determine the relative success of either.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.