ArticleOrganogenesis2016
Par-1b is required for morphogenesis and differentiation of myoepithelial cells during salivary gland development.
Article in Organogenesis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation.Nature communications · 2024Article
- Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype.Acta biomaterialia · 2023Article
- MUC1 and Polarity Markers INADL and SCRIB Identify Salivary Ductal Cells.Journal of dental research · 2022Article
- Maternal Fluoride Exposure Exerts Different Toxicity Patterns in Parotid and Submandibular Glands of Offspring Rats.International journal of molecular sciences · 2022Article
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Regulation of myoepithelial differentiation.PloS one · 2022Article
- Methylmercury-Induced Toxicopathologic Findings in Salivary Glands of Offspring Rats After Gestational and Lactational Exposure.Biological trace element research · 2021Article
- ROCK inhibitor increases proacinar cells in adult salivary gland organoids.Stem cell research · 2019Article
- Epithelial Cell Lineage and Signaling in Murine Salivary Glands.Journal of dental research · 2019Review
- Review
- Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment.American journal of physiology. Regulatory, integrative and comparative physiology · 2018Article
- Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance.Scientific reports · 2018Article
- Functional characterization and genomic studies of a novel murine submandibular gland epithelial cell line.PloS one · 2018Article
- Basement Membranes in Development and Disease.Current topics in developmental biology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The salivary epithelium initiates as a solid mass of epithelial cells that are organized into a primary bud that undergoes morphogenesis and differentiation to yield bilayered acini consisting of interior secretory acinar cells that are surrounded by contractile myoepithelial cells in mature salivary glands. How the primary bud transitions into acini has not been previously documented. We document here that the outer epithelial cells subsequently undergo a vertical compression as they express smooth muscle α-actin and differentiate into myoepithelial cells. The outermost layer of polarized epithelial cells assemble and organize the basal deposition of basement membrane, which requires basal positioning of the polarity protein, Par-1b. Whether Par-1b is required for the vertical compression and differentiation of the myoepithelial cells is unknown. Following manipulation of Par-1b in salivary gland organ explants, Par-1b-inhibited explants showed both a reduced vertical compression of differentiating myoepithelial cells and reduced levels of smooth muscle α-actin. Rac1 knockdown and inhibition of Rac GTPase function also inhibited branching morphogenesis. Since Rac regulates cellular morphology, we investigated a contribution for Rac in myoepithelial cell differentiation. Inhibition of Rac GTPase activity showed a similar reduction in vertical compression and smooth muscle α-actin levels while decreasing the levels of Par-1b protein and altering its basal localization in the outer cells. Inhibition of ROCK, which is required for basal positioning of Par-1b, resulted in mislocalization of Par-1b and loss of vertical cellular compression, but did not significantly alter levels of smooth muscle α-actin in these cells. Overexpression of Par-1b in the presence of Rac inhibition restored basement membrane protein levels and localization. Our results indicate that the basal localization of Par-1b in the outer epithelial cells is required for myoepithelial cell compression, and Par-1b is required for myoepithelial differentiation, regardless of its localization.
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Registered trials
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