ArticleMolecular medicine reports2016
Effects of inactivated Enterococcus faecalis on the proliferation and osteogenic induction of osteoblasts.
Article in Molecular medicine reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.PLoS pathogens · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aimed to evaluate the effects of Enterococcus faecalis, inactivated by the common intracanal irrigants sodium hypochlorite (NaOCl) and chlorhexidine (CHX), on osteoblasts. E. faecalis was inactivated with 2% CHX or 5.25% NaOCl. Subsequently, the Cell Counting kit‑8 assay was used to examine the effects of CHX‑ and NaOCl-inactivated E. faecalis on MC3T3‑E1 osteoblast cell proliferation. Alizarin red staining was used to determine osteoblast mineralization, and osteogenic induction was quantified by determining the optical density of the dye solution. The relative expression levels of osteogenic genes were detected after 1, 4, 7 and 14 days of stimulation with CHX‑ and NaOCl-inactivated E. faecalis by reverse transcription‑quantitative polymerase chain reaction. The results indicated that CHX‑inactivated E. faecalis inhibited osteoblast proliferation, whereas NaOCl‑inactivated E. faecalis did not suppress cell proliferation. Various concentrations of CHX‑ and NaOCl‑inactivated E. faecalis induced different degrees of osteoblast mineralization. The expression levels of osteocalcin, alkaline phosphatase, runt‑related transcription factor 2, osteopontin and osterix were upregulated in cells following stimulation with 107 and 105 colony‑forming units/ml E. faecalis inactivated by CHX and NaOCl; the upregulation of these osteogenic genes occurred at various time points. In conclusion, the present study demonstrated that CHX‑inactivated E. faecalis exerted more of an effect on osteoblast proliferation compared with NaOCl‑inactivated E. faecalis. In addition, CHX‑ and NaOCl‑inactivated E. faecalis was able to induce mineralization and relevant osteogenic gene expression in osteoblast cells.
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