ArticleActa pharmacologica Sinica2017
Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment.
Article in Acta pharmacologica Sinica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 42 citations in OpenAlex.
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- Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs.Veterinary research · 2024Article
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- Isolation and Identification of Two Clinical Strains of the Novel GenotypeMicrobiology spectrum · 2022Article
- Natural products for infectious microbes and diseases: an overview of sources, compounds, and chemical diversities.Science China. Life sciences · 2022Review
- Emodin from Aloe inhibits Swine acute diarrhea syndrome coronavirus in cell culture.Frontiers in veterinary science · 2022Article
- Promising Role of Emodin as Therapeutics to Against Viral Infections.Frontiers in pharmacology · 2022Review
- Advances in the study of emodin: an update on pharmacological properties and mechanistic basis.Chinese medicine · 2021Review
- Emodin fromViruses · 2021Article
- Honeysuckle Aqueous Extracts InducedViruses · 2021Article
- Broad Anti-Viral Capacities of Lian-Hua-Qing-Wen Capsule and Jin-Hua-Qing-Gan Granule and Rational use Against COVID-19 Based on Literature Mining.Frontiers in pharmacology · 2021Article
- DEAD-Box Helicase DDX6 Facilitated RIG-I-Mediated Type-I Interferon Response to EV71 Infection.Frontiers in cellular and infection microbiology · 2021Article
- The Inhibitory Activity of Anthraquinones against Pathogenic Protozoa, Bacteria, and Fungi and the Relationship to Structure.Molecules (Basel, Switzerland) · 2020Article
- An Evaluation of Traditional Persian Medicine for the Management of SARS-CoV-2.Frontiers in pharmacology · 2020Review
- Coxsackievirus A6 Induces Necroptosis for Viral Production.Frontiers in microbiology · 2020Article
- EV71 Infection Induces IFNβ Expression in Neural Cells.Viruses · 2019Article
- Emodin alleviates alternatively activated macrophage and asthmatic airway inflammation in a murine asthma model.Acta pharmacologica Sinica · 2018Article
- Pseudolaric acid B induced autophagy, but not apoptosis, in MRC5 human fibroblast cells.Oncology letters · 2018Article
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Authors and funding
7 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Human enterovirus 71 (EV71) is the primary causative agent of recent large-scale outbreaks of hand, foot, and mouth disease (HFMD) in Asia. Currently, there are no drugs available for the prevention and treatment of HFMD. In this study, we compared the anti-EV71 activities of three natural compounds, rheum emodin, artemisinin and astragaloside extracted from Chinese herbs Chinese rhubarb, Artemisia carvifolia and Astragalus, respectively, which have been traditionally used for the treatment and prevention of epidemic diseases. Human lung fibroblast cell line MRC5 was mock-infected or infected with EV71, and treated with drugs. The cytotoxicity of the drugs was detected with MTT assay. The cytopathic effects such as cell death and condensed nuclei were morphologically observed. The VP1-coding sequence required for EV71 genome replication was assayed with qRT-PCR. Viral protein expression was analyzed with Western blotting. Viral TCID50 was determined to evaluate EV71 virulence. Flow cytometry analysis of propidium iodide staining was performed to analyze the cell cycle distribution of MRC5 cells. Rheum emodin (29.6 μmol/L) effectively protected MRC5 cells from EV71-induced cytopathic effects, which resulted from the inhibiting viral replication: rheum emodin treatment decreased viral genomic levels by 5.34-fold, viral protein expression by less than 30-fold and EV71 virulence by 0.33107-fold. The fact that inhibition of rheum emodin on viral virulence was much stronger than its effects on genomic levels and viral protein expression suggested that rheum emodin inhibited viral maturation. Furthermore, rheum emodin treatment markedly diminished cell cycle arrest at S phase in MRC5 cells, which was induced by EV71 infection and favored the viral replication. In contrast, neither astragaloside (50 μmol/L) nor artemisinin (50 μmol/L) showed similar anti-EV71 activities. Among the three natural compounds tested, rheum emodin effectively suppressed EV71 viral replication, thus is a candidate anti-HFMD drug.
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