ArticleCell systems2016
Design and Construction of Generalizable RNA-Protein Hybrid Controllers by Level-Matched Genetic Signal Amplification.
Article in Cell systems, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Non-invasive single cell aptasensing in live cells and animals.Chemical science · 2024Article
- Review
- Recent trends in biocatalysis.Chemical Society reviews · 2021Review
- A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors.Nature communications · 2021Article
- Massively parallel RNA device engineering in mammalian cells with RNA-Seq.Nature communications · 2019Article
- Prospects for engineering dynamic CRISPR-Cas transcriptional circuits to improve bioproduction.Journal of industrial microbiology & biotechnology · 2018Review
- Dynamic metabolic control: towards precision engineering of metabolism.Journal of industrial microbiology & biotechnology · 2018Article
- Biomedical Applications of RNA-Based Devices.Current opinion in biomedical engineering · 2017Article
- Computational design of small transcription activating RNAs for versatile and dynamic gene regulation.Nature communications · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
For synthetic biology applications, protein-based transcriptional genetic controllers are limited in terms of orthogonality, modularity, and portability. Although ribozyme-based switches can address these issues, their current two-stage architectures and limited dynamic range hinder their broader incorporation into systems-level genetic controllers. Here, we address these challenges by implementing an RNA-protein hybrid controller with a three-stage architecture that introduces a transcription-based amplifier between an RNA sensor and a protein actuator. To facilitate the construction of these more complex circuits, we use a model-guided strategy to efficiently match the activities of stages. The presence of the amplifier enabled the three-stage controller to have up to 200-fold higher gene expression than its two-stage counterpart and made it possible to implement higher-order controllers, such as multilayer Boolean logic and feedback systems. The modularity inherent in the three-stage architecture along with the sensing flexibility of RNA devices presents a generalizable framework for designing and building sophisticated genetic control systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.