ArticleMolecular cell2016
3D-CLEM Reveals that a Major Portion of Mitotic Chromosomes Is Not Chromatin.
Article in Molecular cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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Who cites it
71 citing papers in PubMed, 139 citations in OpenAlex.
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- Ki-67 regulates heterochromatin organization in neurons and forms condensates with heterochromatic components.Nature communications · 2026Article
- Uncovering mitotic ultrastructure in the native hair follicle using volume electron microscopy.Journal of cell science · 2026Article
- Bayesian data driven modelling of kinetochore dynamics: Space-time organisation of the human metaphase plate.PLoS computational biology · 2026Article
- Spatial Chromatin Organization Across the Cell Cycle: Insights from Auxin-Inducible Protein Depletion.Cells · 2025Review
- Pre-rRNAs control mitosis by maintaining chromosomal segregation through protecting SMC2 from AURKA-mediated phosphorylation.Cell death & disease · 2025Article
- Origin of chromosome 12 trisomy surge in human induced pluripotent stem cells.The Journal of cell biology · 2025Article
- Progressive chromosome shape changes during cell divisions.EMBO reports · 2025Article
- B1 corrected T1 mapping in the differentiation and prediction of breast cancer.Scientific reports · 2025Article
- Chromosomal tethering and mitotic transcription promote ecDNA nuclear inheritance.Molecular cell · 2025Article
- Abnormal nucleoli architecture and aggregate formation in nucleophosmin mutated acute myeloid leukaemia.Journal of cell science · 2025Article
- Nanoscale analysis of human G1 and metaphase chromatin in situ.The EMBO journal · 2025Article
- Rules of engagement for condensins and cohesins guide mitotic chromosome formation.Science (New York, N.Y.) · 2025Article
- The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation.Nature communications · 2025Article
- One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.The Journal of cell biology · 2025Article
- Near millimolar concentration of nucleosomes in mitotic chromosomes from late prometaphase into anaphase.The Journal of cell biology · 2024Article
- Orientation-independent-DIC imaging reveals that a transient rise in depletion attraction contributes to mitotic chromosome condensation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology.bioRxiv : the preprint server for biology · 2024Article
- Advanced environmental scanning electron microscopy reveals natural surface nano-morphology of condensed mitotic chromosomes in their native state.Scientific reports · 2024Article
- U3 snoRNA inter-regulates with DDX21 in the perichromosomal region to control mitosis.Cell death & disease · 2024Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Recent studies have revealed the importance of Ki-67 and the chromosome periphery in chromosome structure and segregation, but little is known about this elusive chromosome compartment. Here we used correlative light and serial block-face scanning electron microscopy, which we term 3D-CLEM, to model the entire mitotic chromosome complement at ultra-structural resolution. Prophase chromosomes exhibit a highly irregular surface appearance with a volume smaller than metaphase chromosomes. This may be because of the absence of the periphery, which associates with chromosomes only after nucleolar disassembly later in prophase. Indeed, the nucleolar volume almost entirely accounts for the extra volume found in metaphase chromosomes. Analysis of wild-type and Ki-67-depleted chromosomes reveals that the periphery comprises 30%-47% of the entire chromosome volume and more than 33% of the protein mass of isolated mitotic chromosomes determined by quantitative proteomics. Thus, chromatin makes up a surprisingly small percentage of the total mass of metaphase chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.