Evidence map›Paper›PMID 27840020›Full record

ArticleCell stem cell2017

Functional Human Oocytes Generated by Transfer of Polar Body Genomes.

Hong Ma, Ryan C O'Neil, Nuria Marti Gutierrez, Manoj Hariharan, Zhuzhu Z Zhang, Yupeng He, Cengiz Cinnioglu, Refik Kayali, Eunju Kang, Yeonmi Lee and 16 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
9.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 90 citations in OpenAlex.

  1. Trial
  2. Review
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  4. Mechanistic study on the role of multi-pathway autophagy in ovarian aging: literature review.Apoptosis : an international journal on programmed cell death · 2025
    Review
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  19. Article
  20. The Future of IVF: The New Normal in Human Reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 4 institutions in 1 country.

Hong MaCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Ryan C O'NeilGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Bioinformatics Program, University of California at San Diego, La Jolla, CA 92093, USA.
Nuria Marti GutierrezCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Manoj HariharanGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Zhuzhu Z ZhangGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Yupeng HeGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Bioinformatics Program, University of California at San Diego, La Jolla, CA 92093, USA.
Cengiz CinniogluIviGen Los Angeles, Torrance, CA 90501, USA.
Refik KayaliIviGen Los Angeles, Torrance, CA 90501, USA.
Eunju KangCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Yeonmi LeeCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Tomonari HayamaCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Amy KoskiCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Joseph NeryGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Rosa CastanonGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Rebecca Tippner-HedgesCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Riffat AhmedCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Crystal Van DykenCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Ying LiCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Susan OlsonDepartment of Molecular and Medical Genetics, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
David BattagliaDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA.
David M LeeDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA.
Diana H WuDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA.
Paula AmatoDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA.
Don P WolfCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Joseph R EckerGenomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: ecker@salk.edu.
Shoukhrat MitalipovCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA; Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address: mitalipo@ohsu.edu.
Oregon Health & Science University · USSalk Institute for Biological Studies · USEpigen Biosciences (United States) · USUniversity of California, San Diego · US

Funding

Howard Hughes Medical Institute
6 · The paper itself

Abstract

Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enucleated donor MII cytoplasm (PBNT). The reconstructed oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis completion and formation of normal diploid zygotes. While PBNT zygotes developed to blastocysts less frequently (42%) than controls (75%), genome-wide genetic, epigenetic, and transcriptional analyses of PBNT and control ESCs indicated comparable numbers of structural variations and markedly similar DNA methylation and transcriptome profiles. We conclude that rescue of PB1 genetic material via introduction into donor cytoplasm may offer a source of oocytes for infertility treatment or mitochondrial replacement therapy for mtDNA disease.

Indexed as

Genome, HumanNuclear Transfer TechniquesAdultBlastocystDNA MethylationEmbryonic DevelopmentEpigenesis, GeneticFemaleFertilization in VitroGene Expression ProfilingGenomic InstabilityHuman Embryonic Stem CellsHumansMaleMetaphaseOocyteshaploidinfertility treatmentmeiosismitochondrial replacement therapypolar body nuclear transferreconstructed human oocytes

Identifiers

PMID27840020
PMCPMC5218919
OpenAlexW2553697803

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.