ArticleMethods in molecular biology (Clifton, N.J.)2017
Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.
Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The study of plasmodesmal biology using proximity labeling technologies.Journal of experimental botany · 2026Review
- Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN.PLoS pathogens · 2025Article
- Measuring Plasmodesmata-mediated Intercellular Trafficking Using Microparticle Bombardment in Arabidopsis and Crops.Current protocols · 2025Article
- The disordered effector RipAO of Ralstonia solanacearum destabilizes microtubule networks in Nicotiana benthamiana cells.Molecules and cells · 2025Article
- Identification of WRKY gene family inPeerJ · 2024Article
- The NEL Family of Bacterial E3 Ubiquitin Ligases.International journal of molecular sciences · 2022Review
- Bioinformatics Analysis and Functional Characterization of the CFEM Proteins ofJournal of fungi (Basel, Switzerland) · 2022Article
- Nuclear Localization of HopA1Plants (Basel, Switzerland) · 2021Article
- Computational Systems Biology of Alfalfa - Bacterial Blight Host-Pathogen Interactions: Uncovering the Complex Molecular Networks for Developing Durable Disease Resistant Crop.Frontiers in plant science · 2021Article
- Identification and Functional Analysis of AopN, anInternational journal of molecular sciences · 2020Article
- Pathogenic Bacteria Target Plant Plasmodesmata to Colonize and Invade Surrounding Tissues.The Plant cell · 2020Article
- Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection.Journal of visualized experiments : JoVE · 2018Article
- Spatiotemporal Monitoring ofThe Plant cell · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector systems are a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors that target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular basis of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.