ArticleProceedings of the National Academy of Sciences of the United States of America2016
Two proofreading steps amplify the accuracy of genetic code translation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Error-prone translation as a driver of proteostasis collapse and neurodegeneration.Neural regeneration research · 2026Article
- Translation fidelity mechanisms and their changes in aging.bioRxiv : the preprint server for biology · 2026Article
- RNAViruses · 2026Review
- Direct observation of ATP-driven ubiquitin chain handling by Cdc48.Nature communications · 2026Article
- Structural mechanism of mRNA decoding by mammalian GTPase GTPBP1.Nature communications · 2025Article
- Unchecked growth: Pushing the limits on RNA virus genome size in the absence of known proofreading.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers.Chemical reviews · 2024Review
- Extensive breaking of genetic code degeneracy with non-canonical amino acids.Nature communications · 2023Article
- Bridging ribosomal synthesis to cell growth through the lens of kinetics.Biophysical journal · 2023Article
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- Article
- The Structural Dynamics of Translation.Annual review of biochemistry · 2022Review
- Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation.The EMBO journal · 2021Article
- Mechanistic insights into translation inhibition by aminoglycoside antibiotic arbekacin.Nucleic acids research · 2021Article
- Proofreading through spatial gradients.eLife · 2020Article
- Article
- Elongation factor-Tu can repetitively engage aminoacyl-tRNA within the ribosome during the proofreading stage of tRNA selection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Allostery and Kinetic Proofreading.The journal of physical chemistry. B · 2019Article
- The Reverse Side of a Coin: "Factor-Free" Ribosomal Protein Synthesis In Vitro is a Consequence of the In Vivo Proofreading Mechanism.Biomolecules · 2019Article
- Ribosome Structure, Function, and Early Evolution.International journal of molecular sciences · 2018Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Aminoacyl-tRNAs (aa-tRNAs) are selected by the messenger RNA programmed ribosome in ternary complex with elongation factor Tu (EF-Tu) and GTP and then, again, in a proofreading step after GTP hydrolysis on EF-Tu. We use tRNA mutants with different affinities for EF-Tu to demonstrate that proofreading of aa-tRNAs occurs in two consecutive steps. First, aa-tRNAs in ternary complex with EF-Tu·GDP are selected in a step where the accuracy increases linearly with increasing aa-tRNA affinity to EF-Tu. Then, following dissociation of EF-Tu·GDP from the ribosome, the accuracy is further increased in a second and apparently EF-Tu-independent step. Our findings identify the molecular basis of proofreading in bacteria, highlight the pivotal role of EF-Tu for fast and accurate protein synthesis, and illustrate the importance of multistep substrate selection in intracellular processing of genetic information.
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