ArticleApplied and environmental microbiology2017
Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase.
Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- Semi-Rational Engineering of Formate Dehydrogenase Reveals Loop Dynamics as a Key Determinant of Thermostability.Chembiochem : a European journal of chemical biology · 2026Article
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- Effect of Free Cysteine Residues to Serine Mutation on Cellodextrin Phosphorylase.Journal of applied glycoscience · 2024Article
- Constructing Nanocaged Enzymes for Synergistic Catalysis of COAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
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- Improving Both the Thermostability and Catalytic Efficiency of Phospholipase D fromInternational journal of molecular sciences · 2022Article
- In Silico Analysis of Glucose Oxidase fromBioengineering (Basel, Switzerland) · 2021Article
- Enhanced Prodigiosin Production inApplied and environmental microbiology · 2021Article
- Effect of Met/Leu substitutions on the stability of NAD+-dependent formate dehydrogenases from Gossypium hirsutum.Applied microbiology and biotechnology · 2021Article
- Expression of NovelFrontiers in bioengineering and biotechnology · 2021Article
- Expression and Refolding of the Plant Chitinase FromFrontiers in bioengineering and biotechnology · 2021Article
- Enhancement of Pharmaceutical Urate Oxidase Thermostability by Rational Design ofIranian journal of biotechnology · 2020Article
- Engineered disulfide bonds improve thermostability and activity of L-isoleucine hydroxylase for efficient 4-HIL production inEngineering in life sciences · 2020Article
- A Novel 3-Phytosterone-9α-Hydroxylase Oxygenation Component and Its Application in Bioconversion of 4-Androstene-3,17-Dione to 9α-Hydroxy-4-Androstene-3,17-Dione Coupling with A NADH Regeneration Formate Dehydrogenase.Molecules (Basel, Switzerland) · 2019Article
- Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440.Microbial cell factories · 2019Article
- Characterization of a novel thermotolerant NAD3 Biotech · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
nad importanceFDH is widely used for NADH regeneration in dehydrogenase-based synthesis of optically active compounds to decrease the cost of production. This study highlighted a viable strategy that was used to eliminate the oxidation of free cysteine residues of FDH from Candida boidinii by the introduction of disulfide bonds. Using this strategy, we obtained a variant FDH with improved activity and stability. The improvement of activity and stability of FDH is expected to reduce its price and then further to decrease the cost of its application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.