Evidence map›Paper›PMID 27836841›Full record

ArticleApplied and environmental microbiology2017

Zn2+-Inducible Expression Platform for Synechococcus sp. Strain PCC 7002 Based on the smtA Promoter/Operator and smtB Repressor.

Adam A Pérez, John P Gajewski, Bryan H Ferlez, Marcus Ludwig, Carol S Baker, John H Golbeck, Donald A Bryant

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Regulatory Tools for Controlling Gene Expression in Cyanobacteria.Advances in experimental medicine and biology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Adam A PérezDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
John P GajewskiDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Bryan H FerlezDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Marcus LudwigDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Carol S BakerDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
John H GolbeckDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Donald A BryantDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA dab14@psu.edu.ORCID http://orcid.org/0000-0003-2782-5988
Pennsylvania State University · US

Funding

DROSOPHILA GENOMICS RESOURCE CENTERP40OD010949 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Andrew Zelhof · 2012 to 2026
$9.4M
NIH HHS P40 OD010949
6 · The paper itself

Abstract

Synechococcus sp. strain PCC 7002 has been gaining significance as both a model system for photosynthesis research and for industrial applications. Until recently, the genetic toolbox for this model cyanobacterium was rather limited and relied primarily on tools that only allowed constitutive gene expression. This work describes a two-plasmid, Zn IMPORTANCE: This is the first metal-responsive expression system in cyanobacteria, to our knowledge, that does not exhibit low sensitivity for induction, which is one of the major hurdles for utilizing this class of genetic tools. In addition, high levels of expression can be generated that approximate those of established constitutive systems, with the added advantage of titratable control. Together, these properties establish this Zn

Indexed as

Gene Expression Regulation, BacterialOperator Regions, GeneticPromoter Regions, GeneticAnimalsAnimals, Genetically ModifiedBacterial ProteinsDrosophila melanogasterMetallothioneinSynechococcusBacterial ProteinsMetallothioneinsmtA protein, Synechococcuscyanobacteriagene expression platformmetallothioneinninaBphotosynthesisZn2+-inducible promoter

Identifiers

PMID27836841
PMCPMC5244298
OpenAlexW2556120610

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.