ArticleBMC genomics2016
Precise mapping of the transcription start sites of human microRNAs using DROSHA knockout cells.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- MicroRNA-375-3p Targets Fatty Acid Synthase and Relish to Regulate Energy Allocation During Pupal Metamorphosis and Starvation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquitoProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquitoProceedings of the National Academy of Sciences of the United States of America · 2021Article
- NRF2 is a key regulator of endothelial microRNA expression under proatherogenic stimuli.Cardiovascular research · 2021Article
- MicroRNA-1 Expression and Function inFrontiers in physiology · 2021Article
- Uncovering the cellular capacity for intensive and specific feedback self-control of the argonautes and MicroRNA targeting activity.Nucleic acids research · 2020Article
- Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension.Nature communications · 2020Article
- Article
- NRF2 regulates endothelial glycolysis and proliferation with miR-93 and mediates the effects of oxidized phospholipids on endothelial activation.Nucleic acids research · 2018Article
- Emerging roles of DROSHA beyond primary microRNA processing.RNA biology · 2018Article
- Article
- Knockout of miR-221 and miR-222 reveals common and specific targets for paralogous miRNAs.RNA biology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe expression of microRNAs (miRNAs) is primarily regulated during their transcription. However, the transcriptional regulation of miRNA genes has not been studied extensively owing to the lack of sufficient information about the promoters and transcription start sites of most miRNAs.
resultsIn this study, we identified the transcription start sites of human primary miRNAs (pri-miRNAs) using DROSHA knockout cells. DROSHA knockout resulted in increased accumulation of pri-miRNAs and facilitated the precise mapping of their 5' end nucleotides using the rapid amplification of cDNA ends (RACE) technique. By analyzing the promoter region encompassing the transcription start sites of miRNAs, we found that the unrelated miRNAs in their sequences have many common elements in their promoters for binding the same transcription factors. Moreover, by analyzing intronic miRNAs, we also obtained comprehensive evidence that miRNA-harboring introns are spliced more slowly than other introns.
conclusionsThe precisely mapped transcription start sites of pri-miRNAs, and the list of transcription factors for pri-miRNAs regulation, will be valuable resources for future studies to understand the regulatory network of miRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.