ArticleThe Journal of clinical investigation1989
Osteoclast-like cells form in long-term human bone marrow but not in peripheral blood cultures.
Article in The Journal of clinical investigation, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 76 citations in OpenAlex.
- From Development, Disease, and Decline: A Review of What Defines an Osteoclast Progenitor.International journal of molecular sciences · 2025Review
- UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis.Nature communications · 2023Article
- Origin of Osteoclasts: Osteoclast Precursor Cells.Journal of bone metabolism · 2023Article
- Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021Review
- Peripheral blood monocytes show increased osteoclast differentiation potential compared to bone marrow monocytes.Heliyon · 2018Article
- A simplified method for the generation of human osteoclasts in vitro.International journal of biochemistry and molecular biology · 2011Article
- 1-Alpha, 25-dihydroxy vitamin D3 inhibits osteoclastogenesis through IFN-beta-dependent NFATc1 suppression.Journal of bone and mineral metabolism · 2009Article
- Annexin II increases osteoclast formation by stimulating the proliferation of osteoclast precursors in human marrow cultures.The Journal of clinical investigation · 1999Article
- Human blood-mobilized hematopoietic precursors differentiate into osteoclasts in the absence of stromal cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- 1,25-Dihydroxyvitamin D3 promotes prostaglandin E1-induced differentiation of HL-60 cells.Calcified tissue international · 1995Article
- Downregulation of calcitonin receptor mRNA expression by calcitonin during human osteoclast-like cell differentiation.The Journal of clinical investigation · 1995Article
- Osteoclast precursors circulate in avian blood.Calcified tissue international · 1992Article
- An assessment of the ability of human bone marrow cultures to generate osteoclasts.International journal of experimental pathology · 1992Article
- Species differences in the immunophenotype of osteoclasts and mononuclear phagocytes.Calcified tissue international · 1992Article
- Interleukin 6. A potential autocrine/paracrine factor in Paget's disease of bone.The Journal of clinical investigation · 1992Article
- A quantitative cytochemical investigation of osteoclasts and multinucleate giant cells.The Histochemical journal · 1991Article
- 1 alpha,25-dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Osteoinductive factor inhibits formation of human osteoclast-like cells.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Atypical multinucleated cells form in long-term marrow cultures from patients with Paget's disease.The Journal of clinical investigation · 1990Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Transplantation studies have suggested that peripheral blood mononuclear cells contain precursors for osteoclasts. Thus we tested the capacity of peripheral blood monocytes to form osteoclasts in long-term culture. We have reported previously that mononuclear cells from feline, baboon, and human marrow form osteoclast-like cells in long term cultures. Further, the formation of these cells is increased in response to bone resorption stimulatory agents such as PTH, interleukin 1, and transforming growth factor alpha. We now report that these cells show characteristic cytoplasmic contraction with calcitonin and form resorption lacunae when cultured on sperm whale dentine. Thus, these bone marrow-derived multinucleated cells fulfill the functional criteria for osteoclasts. Although cultured peripheral blood monocytes can be induced to form multinucleated cells with 1,25-dihydroxyvitamin D3, these cells did not show similar responses to the osteotropic factors as multinucleated cells formed in the bone marrow cultures multinucleated cells. These results indicate that osteoclasts or cells closely related to osteoclasts form in long-term human bone marrow cultures. In contrast, few mononuclear cells in the peripheral blood appear capable of forming osteoclasts under the culture conditions used in these experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.