Evidence map›Paper›PMID 27834745›Full record

ArticleStroke2016

Altered Expression of Long Noncoding RNAs in Blood After Ischemic Stroke and Proximity to Putative Stroke Risk Loci.

Cheryl Dykstra-Aiello, Glen C Jickling, Bradley P Ander, Natasha Shroff, Xinhua Zhan, DaZhi Liu, Heather Hull, Miles Orantia, Boryana S Stamova, Frank R Sharp

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
89citing papers in PubMed, 2 pooled it
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

89 citing papers in PubMed, 2 syntheses or guidelines pooled it, 135 citations in OpenAlex.

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  4. Molecular heterogeneity in human stroke - What can we learn from the peripheral blood transcriptome?Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026
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29 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Cheryl Dykstra-AielloFrom the Department of Neurology, University of California at Davis, Sacramento.
Glen C JicklingFrom the Department of Neurology, University of California at Davis, Sacramento.
Bradley P AnderFrom the Department of Neurology, University of California at Davis, Sacramento.
Natasha ShroffFrom the Department of Neurology, University of California at Davis, Sacramento.
Xinhua ZhanFrom the Department of Neurology, University of California at Davis, Sacramento.
DaZhi LiuFrom the Department of Neurology, University of California at Davis, Sacramento.
Heather HullFrom the Department of Neurology, University of California at Davis, Sacramento.
Miles OrantiaFrom the Department of Neurology, University of California at Davis, Sacramento.
Boryana S StamovaFrom the Department of Neurology, University of California at Davis, Sacramento. bsstamova@ucdavis.edu.
Frank R SharpFrom the Department of Neurology, University of California at Davis, Sacramento.
University of California, Davis · US

Funding

Molecular Biology of Stroke in HumansR01NS075035 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R · 2012 to 2016
$3.1M
Whole Genome RNA Sequencing (RNAseq) of Blood from Patients with Lacunar StrokeR01NS101718 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R, STAMOVA, BORYANA · 2017 to 2021
$3.0M
Whole Transcriptome Studies of Patients with Transient Ischemic Attacks (TIAs)R01NS097000 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R, STAMOVA, BORYANA · 2016 to 2020
$2.8M
A Novel Approach to Assessing Cryptogenic StrokeR01NS079153 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R · 2012 to 2016
$2.8M
Role of the Src Family Kinases in Traumatic Brain InjuryR01NS089901 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LIU, DA ZHI · 2015 to 2019
$1.7M
NINDS NIH HHS R01 NS075035NINDS NIH HHS R01 NS079153NINDS NIH HHS R01 NS089901NINDS NIH HHS R01 NS097000NINDS NIH HHS R01 NS101718
6 · The paper itself

Abstract

background and purposeAlthough peripheral blood mRNA and micro-RNA change after ischemic stroke, any role for long noncoding RNA (lncRNA), which comprise most of the genome and have been implicated in various diseases, is unknown. Thus, we hypothesized that lncRNA expression also changes after stroke.

methodslncRNA expression was assessed in 266 whole-blood RNA samples drawn once per individual from patients with ischemic stroke and matched with vascular risk factor controls. Differential lncRNA expression was assessed by ANCOVA (P<0.005; fold change>|1.2|), principal components analysis, and hierarchical clustering on a derivation set (n=176) and confirmed on a validation set (n=90). Poststroke temporal lncRNA expression changes were assessed using ANCOVA with confounding factor correction (P<0.005; partial correlation with time since event >|0.4|). Because sexual dimorphism exists in stroke, analyses were performed for each sex separately.

resultsA total of 299 lncRNAs were differentially expressed between stroke and control males, whereas 97 lncRNAs were differentially expressed between stroke and control females. Significant changes of lncRNA expression with time after stroke were detected for 49 lncRNAs in men and 31 lncRNAs in women. Some differentially expressed lncRNAs mapped close to genomic locations of previously identified putative stroke-risk genes, including lipoprotein, lipoprotein(a)-like 2, ABO (transferase A, α1-3-N-acetylgalactosaminyltransferase; transferase B, α1-3-galactosyltransferase) blood group, prostaglandin 12 synthase, and α-adducins.

conclusionsThis study provides evidence of altered and sexually dimorphic lncRNA expression in peripheral blood of patients with stroke compared with that of controls and suggests that lncRNAs have potential for stroke biomarker development. Some regulated lncRNA could regulate some previously identified putative stroke-risk genes.

Indexed as

AdultAgedAged, 80 and overBiomarkersBrain IschemiaFemaleGene Expression RegulationGenetic LociHumansMaleMiddle AgedRisk FactorsRNA, Long NoncodingStrokeTime FactorsBiomarkersRNA, Long Noncodinggene expressionrisk factorRNA, long noncodingRNA, untranslatedstroke

Identifiers

PMID27834745
PMCPMC5127755
OpenAlexW2549104904

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.