ArticleThe Indian journal of medical research2016
A novel indirect ELISA for diagnosis of dengue fever.
Article in The Indian journal of medical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Analyzing the Molecular Signature Genes and Pathways of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome Caused by Dengue Virus in India.Molecular biotechnology · 2026Article
- Preparation of FeMikrochimica acta · 2025Article
- Identification of visible and near-infrared signature peaks for arboviruses and Plasmodium falciparum.PloS one · 2025Article
- Article
- Article
- A Critical Analysis of Intracranial Hemorrhage as a Fatal Complication of Dengue Fever.Journal of Korean Neurosurgical Society · 2023Article
- Review
- Diagnosis of dengue virus infection using spectroscopic images and deep learning.PeerJ. Computer science · 2022Article
- Dengue Detection: Advances in Diagnostic Tools from Conventional Technology to Point of Care.Biosensors · 2021Review
- Linear and Continuous Flavivirus Epitopes From Naturally Infected Humans.Frontiers in cellular and infection microbiology · 2021Review
- Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections.Viruses · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesDengue fever (DF) is associated with significant morbidity and mortality in the tropical and sub-tropical regions of the world. Since there are no effective antiviral drugs for treatment, clinicians often rely on the accurate diagnosis of dengue fever to begin supportive therapy at early stages of the illness. The objective of this study was to develop an in-house dengue virus serotype 2 (DENV-2) non-structural protein- 5 (NS5) based indirect ELISA.
methodsDENV-2 was raised in Vero cells and the viral proteins were separated and subsequently the NS5 protein was eluted. Serum samples from primary and secondary dengue fever patients; and acute and convalescent samples from Japanese encephalitis (JE) and West Nile virus (WNV) cases were used to validate the ELISA.
resultsThe assay was found to be 100 per cent specific in detecting DENV-2 specific antibodies from patient's serum. However, in terms of sensitivity, the assay could detect IgM antibodies only from 90 per cent of the primary dengue samples. The IgM/IgG ratio of the primary and secondary samples was 7.24 and 0.64, respectively. INTERPRETATION &
conclusionsThe results indicate that the DENV-2 NS5 ELISA is dengue group specific and can be used to differentiate dengue infection from other circulating Flavivirus infections. This NS5 ELISA can also be used to distinguish between primary and secondary dengue fever on the basis of IgM/IgG ratios. Further studies with larger sample sizes and different DENV serotypes are required to validate the ELISA.
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