ArticleJournal of analytical methods in chemistry2016
Assessment for Melting Temperature Measurement of Nucleic Acid by HRM.
Article in Journal of analytical methods in chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
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- Preparation of topoisomers of short circular dsDNA with defined linking number by accurate topological control.Nucleic acids research · 2025Article
- High-Resolution Melting PCR as a Fast and Simple Molecular Biology-Based Method for the Identification of HypervirulentMicroorganisms · 2024Article
- Harnessing noncanonical crRNA for highly efficient genome editing.Nature communications · 2024Article
- Cytosine methylation regulates DNA bendability depending on the curvature.Chemical science · 2022Article
- Nonalternating purine pyrimidine sequences can form stable left-handed DNA duplex by strong topological constraint.Nucleic acids research · 2022Article
- Developing predictive hybridization models for phosphorothioate oligonucleotides using high-resolution melting.PloS one · 2022Article
- Incorporation of β-Alanine in Cu(II) ATCUN Peptide Complexes Increases ROS Levels, DNA Cleavage and Antiproliferative Activity.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Hybridization-mediated off-target effects of splice-switching antisense oligonucleotides.Nucleic acids research · 2020Article
- Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures.BMC research notes · 2019Article
- RNA ligation of very small pseudo nick structures by T4 RNA ligase 2, leading to efficient production of versatile RNA rings.RSC advances · 2019Article
- Terminal hairpin in oligonucleotide dominantly prioritizes intramolecular cyclization by T4 ligase over intermolecular polymerization: an exclusive methodology for producing ssDNA rings.Nucleic acids research · 2018Article
- A method to detectCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2018Article
- Cyclization of secondarily structured oligonucleotides to single-stranded rings by usingRSC advances · 2018Article
- Study of polymorphisms in theMolecular vision · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High resolution melting (HRM), with a high sensitivity to distinguish the nucleic acid species with small variations, has been widely applied in the mutation scanning, methylation analysis, and genotyping. For the aim of extending HRM for the evaluation of thermal stability of nucleic acid secondary structures on sequence dependence, we investigated effects of the dye of EvaGreen, metal ions, and impurities (such as dNTPs) on melting temperature (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.