ArticleScientific reports2016
Near-infrared bioluminescent proteins for two-color multimodal imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- PrismaLuc luciferase enables tunable near-infrared bioluminescence forRSC chemical biology · 2026Article
- Review
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
- Biomimetic Exogenous "Tissue Batteries" as Artificial Power Sources for Implantable Bioelectronic Devices Manufacturing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Novel BRET combination for detection of rapamycin-induced protein dimerization using luciferase from fungusHeliyon · 2024Article
- Near-Infrared Optogenetic Module for Conditional Protein Splicing.Journal of molecular biology · 2023Article
- Multiplex quadruple bioluminescent assay system.Scientific reports · 2022Article
- A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing.Biosensors · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Nuclear Localization Signals for Optimization of Genetically Encoded Tools in Neurons.Frontiers in cell and developmental biology · 2022Article
- Brightening up Biology: Advances in Luciferase Systems forACS chemical biology · 2021Review
- Powering Implantable and Ingestible Electronics.Advanced functional materials · 2021Article
- Advances in engineering near-infrared luminescent materials.iScience · 2021Review
- Orthogonal Bioluminescent Probes from Disubstituted Luciferins.Biochemistry · 2021Article
- Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.iScience · 2021Article
- Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.International journal of molecular sciences · 2020Review
- Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.Cell chemical biology · 2020Review
- A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.Nature communications · 2020Article
- BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate.RSC advances · 2019Article
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3 authors.
Funding
Abstract
Bioluminescence imaging became a widely used technique for noninvasive study of biological processes in small animals. Bioluminescent probes with emission in near-infrared (NIR) spectral region confer the advantage of having deep tissue penetration capacity. However, there are a very limited number of currently available luciferases that exhibit NIR bioluminescence. Here, we engineered two novel chimeric probes based on RLuc8 luciferase fused with iRFP670 and iRFP720 NIR fluorescent proteins. Due to an intramolecular bioluminescence resonance energy transfer (BRET) between RLuc8 and iRFPs, the chimeric luciferases exhibit NIR bioluminescence with maxima at 670 nm and 720 nm, respectively. The 50 nm spectral shift between emissions of the two iRFP chimeras enables combined multicolor bioluminescence imaging (BLI) and the respective multicolor fluorescence imaging (FLI) of the iRFPs. We show that for subcutaneously implanted cells, NIR bioluminescence provided a 10-fold increase in sensitivity compared to NIR FLI. In deep tissues, NIR BLI enabled detection of as low as 10
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