ArticleScientific reports2016
Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton.BMC genomics · 2021Article
- Comparative Proteomic Investigation of Plasma Reveals Novel Potential Biomarker Groups for Acute Aortic Dissection.Disease markers · 2020Article
- Comparative Proteomic Analysis of Molecular Differences between Leaves of Wild-Type Upland Cotton and ItsMolecules (Basel, Switzerland) · 2019Article
- An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS.BMC genomics · 2019Article
- Genome-wide investigation and expression profiling of APX gene family in Gossypium hirsutum provide new insights in redox homeostasis maintenance during different fiber development stages.Molecular genetics and genomics : MGG · 2018Article
- Revisiting antithrombotic therapeutics; sculptin, a novel specific, competitive, reversible, scissile and tight binding inhibitor of thrombin.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
On two-dimensional electrophoresis gels, six protein spots from cotton ovules and fibers were identified as heat shock cognate 70 kD protein (Hsc70). Three spots corresponded to an experimental molecular weight (MW) of 70 kD (spots 1, 2 and 3), and the remaining three spots corresponded to an experimental MW slightly greater than 45 kD (spots 4, 5 and 6). Protein spots 1, 2 and 3 were abundant on gels of 0-day (the day of anthesis) wild-type (WT) ovules, 0-day fuzzless-lintless mutant ovules and 10-day WT ovules but absent from gels of 10-day WT fibers. Three individual transcripts encoding these six protein spots were obtained by using rapid amplification of cDNA ends (RACE). Edman degradation and western blotting confirmed that the three 45 kD Hsc70 protein spots had the same N-terminal, which started from the T271 amino acid in the intact Hsc70 protein. Furthermore, quadrupole time-of-flight mass spectrometry analysis identified a methylation modification on the arginine at position 475 for protein spots 4 and 5. Our data demonstrate that site-specific in vivo N-terminal truncation of the Hsc70 protein was particularly prevalent in cotton fibers, indicating that post-translational regulation might play an important role in cotton fiber development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.