Evidence map›Paper›PMID 27832775›Full record

ArticleCardiovascular diabetology2016

Correlation between insulin-induced estrogen receptor methylation and atherosclerosis.

Jia Min, Zhong Weitian, Cai Peng, Peng Yan, Zhang Bo, Wang Yan, Bai Yun, Wang Xukai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular diabetology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jia MinDepartment of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
Zhong WeitianDepartment of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
Cai PengDepartment of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
Peng YanDepartment of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
Zhang BoDepartment of Medical Genetics, College of Basic Medicine, Third Military, Medical University, Chongqing, 400038, China.
Wang YanDepartment of Medical Genetics, College of Basic Medicine, Third Military, Medical University, Chongqing, 400038, China.
Bai YunDepartment of Medical Genetics, College of Basic Medicine, Third Military, Medical University, Chongqing, 400038, China. baiyungene@gmail.com.
Wang XukaiDepartment of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China. wangxuk@163.com.
Army Medical University · CNDaping Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHyperinsulinemia and insulin resistance have been recently recognized as an important cause of atherosclerosis. Clinical studies have also found that expression of the estrogen receptor is closely related to the incidence of atherosclerosis. This study investigate the effects of insulin and estrogen receptor α (ER-α) in atherosclerosis.

methodsDouble knockout ApoE/Lepr mice were given intraperitoneal injections of insulin, and their aortae were harvested for hematoxylin-eosin staining and immunohistochemical analysis. In addition, vascular smooth muscle cells (VSMCs) were treated with insulin or infected with a lentivirus encoding exogenous ER-α, and changes in gene expression were detected by real-time polymerase chain reaction and western blotting. The methylation levels of the ER-α gene were tested using bisulfite sequencing PCR, and flow cytometry and EdU assay were used to measure VSMCs proliferation.

resultsOur results showed that insulin can induce the formation of atherosclerosis. Gene expression analysis revealed that insulin promotes the expression of DNA methyltransferases and inhibits ER-α expression, while 5-aza-2'-deoxycytidine can inhibit this effect of insulin. Bisulfite sequencing PCR analysis showed that methylation of the ER-α second exon region increased in VSMCs treated with insulin. The results also showed that ER-α can inhibit VSMCs proliferation.

conclusionsOur data suggest that insulin promotes the expression of DNA methyltransferases, induces methylation of ER-α second exon region and decreases the expression of ER-α, thereby interfering with estrogen regulation of VSMCs proliferation, resulting in atherosclerosis.

Indexed as

DNA MethylationAnimalsAortaAortic DiseasesApolipoproteins EAtherosclerosisCell LineCell ProliferationDisease Models, AnimalEstrogen Receptor alphaExonsFemaleGenetic Predisposition to DiseaseInsulinMice, KnockoutMuscle, Smooth, VascularApolipoproteins EEstrogen Receptor alphaInsulinReceptors, LeptinDNA methylationEpigeneticsEstrogen receptor (ER)InsulinVSMC

Identifiers

PMID27832775
PMCPMC5105242
OpenAlexW2555479161

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.