Evidence map›Paper›PMID 27832373›Full record

ArticleMedical microbiology and immunology2017

Human polyomavirus and human papillomavirus prevalence and viral load in non-malignant tonsillar tissue and tonsillar carcinoma.

Stephan Herberhold, Martin Hellmich, Marcus Panning, Eva Bartok, Steffi Silling, Baki Akgül, Ulrike Wieland

Abstract read
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In one paragraph

Article in Medical microbiology and immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 2 pooled it
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.

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  20. The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Stephan HerberholdENT Department, Head and Neck Surgery, University of Bonn, Bonn, Germany.
Martin HellmichInstitute of Medical Statistics, Informatics and Epidemiology, University of Cologne, Cologne, Germany.
Marcus PanningInstitute for Virology, Medical Center - University of Freiburg, Freiburg, Germany.
Eva BartokInstitute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, Bonn, Germany.
Steffi SillingInstitute of Virology, National Reference Center for Papilloma- and Polyomaviruses, University of Cologne, Fürst-Pückler-Str. 56, 50935, Cologne, Germany.
Baki AkgülInstitute of Virology, National Reference Center for Papilloma- and Polyomaviruses, University of Cologne, Fürst-Pückler-Str. 56, 50935, Cologne, Germany. baki.akguel@uk-koeln.de.ORCID http://orcid.org/0000-0002-6564-9412
Ulrike WielandInstitute of Virology, National Reference Center for Papilloma- and Polyomaviruses, University of Cologne, Fürst-Pückler-Str. 56, 50935, Cologne, Germany.
University of Cologne · DEUniversity of Bonn · DEUniversity of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human papillomaviruses (HPVs) are an acknowledged cause of a subset of oropharyngeal cancers, especially of tonsillar cancer. Similar to HPV, some human polyomaviruses (HPyVs), such as Merkel cell polyomavirus (MCPyV), have an oncogenic potential. Recently, several novel HPyVs have been discovered. The aim of our study was to determine viral DNA prevalence and viral DNA load of 13 different HPyVs in benign and malignant tonsillar tissue and to compare the data with those found for HPV. A total of 78 biopsies of palatine tonsils with a histologic diagnosis of non-malignant disease (chronic tonsillitis, tonsillar hyperplasia, n = 40) or tonsillar squamous cell carcinoma (n = 38) were included in the study. HPyV DNA prevalence and viral load were determined by virus-specific quantitative real-time PCRs. JCPyV (1/40, 2.5%) and WUPyV (3/40, 7.5%) were only found in non-malignant tonsillar tissue. HPyV7 and HPyV10 were only detected in one (2.6%) and seven (18.4%) of the 38 cancer biopsies, respectively. Both MCPyV (8/38, 21.1 vs. 4/40, 10.0%) and HPyV6 (2/38, 5.3 vs. 1/40, 2.5%) were found more frequently in cancer samples than in non-malignant tissue, but the differences were not significant. BKPyV, KIPyV, TSPyV, HPyV9, STLPyV, HPyV12 and NJPyV were not discovered in any of the samples. HPyV loads found in HPyV DNA-positive biopsies were very low with no difference between non-malignant and malignant samples (median load <0.0001 HPyV DNA copies per beta-globin gene copy, respectively). In contrast to HPyV, high-risk HPV types (HPV16/HPV18) were found significantly more frequently in tonsillar cancers than in non-malignant tonsillar tissue (17/38, 44.7 vs. 2/40, 5.0%, p < 0.001). Furthermore, high-risk HPV DNA loads were significantly higher in the cancer compared to the non-malignant samples (median load 11.861 vs. 7 × 10

Indexed as

PapillomaviridaePolyomavirusAdultAgedBiopsyCarcinomaDNA, ViralFemaleHumansHyperplasiaMaleMiddle AgedPalatine TonsilPapillomavirus InfectionsPolyomavirus InfectionsPrevalenceDNA, ViralHuman papillomavirusHuman polyomavirusTonsillar carcinomaTonsillar hyperplasiaTonsillitis

Identifiers

PMID27832373
OpenAlexW2550166470

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.