ArticleInternational journal of legal medicine2017
A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault.
Article in International journal of legal medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Alternative direct-to-amplification sperm cell lysis techniques for sexual assault sample processing.Journal of forensic sciences · 2022Article
- Rapid oral bacteria detection based on real-time PCR for the forensic identification of saliva.Scientific reports · 2018Article
- Alterations in Human Semen After Infection with SARS-CoV-2: A Meta-analysis.Journal of reproduction & infertilityArticle
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Authors and funding
7 authors.
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Abstract
Sexual assault samples are some of the most common samples encountered in forensic analysis. These samples can require a significant time investment due to differential extraction processes. We report on the first record of successful direct amplification of semen for STR analysis. Neat seminal fluid, dilutions ranging from 1:5 to 1:160 and GEDNAP samples were successfully amplified using a direct method. A mild differential isolation technique to enrich spermatozoa was developed and successfully implemented to separate and directly amplify a mixture of semen and female epithelial cells. Aliquots of samples subjected to the differential isolation protocol were stained with Haemotoxylin and Eosin for sperm scoring. Samples stained after PCR showed a complete lack of intact spermatozoa demonstrating that the cells are lysed during the PCR process. This paper demonstrates the potential to incorporate direct PCR in cases of sexual assault to more rapidly obtain results and achieve a higher sensitivity.
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