ArticleInvestigative ophthalmology & visual science2016
Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Three-Dimensional Culture of Epithelial Cells on Electrospun Nanofibrous Scaffolds.International journal of molecular sciences · 2025Review
- Titaminates, a Biocompatible Titanium-Organic Hybrid Film, Support the Growth and Function of Conjunctival Epithelial Cells.American journal of ophthalmology · 2025Article
- Polymers and Biomaterials for Posterior Lamella of the Eyelid and the Lacrimal System.Polymers · 2024Review
- Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?Bioengineering & translational medicine · 2023Review
- Spatially-Resolved Network Dynamics of Poly(vinyl alcohol) Gels Measured with Dynamic Small Angle Light Scattering.Gels (Basel, Switzerland) · 2022Article
- Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction.Stem cells international · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.Biomaterials · 2021Article
- Scaffolding kidney organoids on silk.Journal of tissue engineering and regenerative medicine · 2019Article
- Conjunctival MUC5AC+ goblet cell index: relationship with corneal nerves and dry eye.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
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Authors and funding
15 authors at 7 institutions in 5 countries.
Funding
Abstract
purposeWe fabricated and investigated polymeric scaffolds that can substitute for the conjunctival extracellular matrix to provide a substrate for autologous expansion of human conjunctival goblet cells in culture.
methodsWe fabricated two hydrogels and two silk films: (1) recombinant human collagen (RHC) hydrogel, (2) recombinant human collagen 2-methacryloylxyethyl phosphorylcholine (RHC-MPC) hydrogel, (3) arginine-glycine-aspartic acid (RGD) modified silk, and (4) poly-D-lysine (PDL) coated silk, and four electrospun scaffolds: (1) collagen, (2) poly(acrylic acid) (PAA), (3) poly(caprolactone) (PCL), and (4) poly(vinyl alcohol) (PVA). Coverslips and polyethylene terephthalate (PET) were used for comparison. Human conjunctival explants were cultured on scaffolds for 9 to 15 days. Cell viability, outgrowth area, and the percentage of cells expressing markers for stratified squamous epithelial cells (cytokeratin 4) and goblet cells (cytokeratin 7) were determined.
resultsMost of cells grown on all scaffolds were viable except for PCL in which only 3.6 ± 2.2% of the cells were viable. No cells attached to PVA scaffold. The outgrowth was greatest on PDL-silk and PET. Outgrowth was smallest on PCL. All cells were CK7-positive on RHC-MPC while 84.7 ± 6.9% of cells expressed CK7 on PDL-silk. For PCL, 87.10 ± 3.17% of cells were CK7-positive compared to PET where 67.10 ± 12.08% of cells were CK7-positive cells.
conclusionsBiopolymer substrates in the form of hydrogels and silk films provided for better adherence, proliferation, and differentiation than the electrospun scaffolds and could be used for conjunctival goblet cell expansion for eventual transplantation once undifferentiated and stratified squamous cells are included. Useful polymer scaffold design characteristics have emerged from this study.
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