ArticlePloS one2016
A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Development and comparative evaluation of TaqMan real-time PCR assays for detecting Enterocytozoon hepatopenaei (EHP) in penaeid shrimp.Scientific reports · 2026Article
- Unlocking the hidden anatomy: synchrotron micro-tomography of the stomach, midgut, and organs in Penaeus vannamei and the potential route of Enterocytozoon hepatopaenaei (EHP) infection.Cell and tissue research · 2026Article
- Identification and full genome characterization of a novel CRESS DNA virus associated with giant freshwater prawn, Macrobrachium Rosenbergii.Archives of virology · 2026Article
- Regional Prevalence and Molecular Detection ofAnimals : an open access journal from MDPI · 2025Article
- Detection and Genetic Characterization ofAnimals : an open access journal from MDPI · 2025Article
- Emergence of decapod hepanhamaparvovirus genotype V and its co-infection withVeterinary world · 2025Article
- Ecytonucleospora hepatopenaei causes lipid droplet depletion and imbalanced lipid metabolism in Penaeus vannamei.Scientific reports · 2025Article
- Simultaneous detection of five shrimp pathogens using a single-tube EvaGreen real-time PCR assay with differential melting temperature.Applied and environmental microbiology · 2025Article
- Article
- Multiplex CRISPR-Cas Assay for Rapid, Isothermal and Visual Detection of White Spot Syndrome Virus (WSSV) and Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimp.Journal of fish diseases · 2025Article
- Metagenomic Studies Reveal the Evidence of Akkermansia muciniphila and Other Probiotic Bacteria in the Gut of Healthy and Enterocytozoon hepatopenaei (EHP)-Infected Farmed Penaeus vannamei.Probiotics and antimicrobial proteins · 2025Article
- Advanced Pathogen Monitoring inViruses · 2025Article
- Article
- Ecytonucleospora hepatopenaei (EHP) disease prevalence and mortality in Litopenaeus vannamei: a comparative study from Eastern India shrimp farms.BMC microbiology · 2024Article
- End-point RPA-CRISPR/Cas12a-based detection of Enterocytozoon bieneusi nucleic acid: rapid, sensitive and specific.BMC veterinary research · 2024Article
- Article
- The Pathogenic Mechanism ofMicroorganisms · 2024Article
- Las Bolitas Syndrome inMicroorganisms · 2024Article
- The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian ParasiteJournal of microbiology and biotechnology · 2024Article
- Shrimp injection with dsRNA targeting the microsporidian EHP polar tube protein reduces internal and external parasite amplification.Scientific reports · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is an important disease of cultivated shrimp. Heavy infections may lead to retarded growth and unprofitable harvests. Existing PCR detection methods target the EHP small subunit ribosomal RNA (SSU rRNA) gene (SSU-PCR). However, we discovered that they can give false positive test results due to cross reactivity of the SSU-PCR primers with DNA from closely related microsporidia that infect other aquatic organisms. This is problematic for investigating and monitoring EHP infection pathways. To overcome this problem, a sensitive and specific nested PCR method was developed for detection of the spore wall protein (SWP) gene of EHP (SWP-PCR). The new SWP-PCR method did not produce false positive results from closely related microsporidia. The first PCR step of the SWP-PCR method was 100 times (104 plasmid copies per reaction vial) more sensitive than that of the existing SSU-PCR method (106 copies) but sensitivity was equal for both in the nested step (10 copies). Since the hepatopancreas of cultivated shrimp is not currently known to be infected with microsporidia other than EHP, the SSU-PCR methods are still valid for analyzing hepatopancreatic samples despite the lower sensitivity than the SWP-PCR method. However, due to its greater specificity and sensitivity, we recommend that the SWP-PCR method be used to screen for EHP in feces, feed and environmental samples for potential EHP carriers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.