ArticlePloS one2016
Impedance Flow Cytometry: A Novel Technique in Pollen Analysis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Challenges and solutions in hand pollination for hybrid pepper (Capsicum annuum L.) seed production: a review.Planta · 2026Review
- Utilization of the litchi male-sterile line 'MS1'-assisting in the selection of suitable harvesting periods for pollen donors for hybridization.Frontiers in plant science · 2026Article
- Impedance flow cytometry for rapid quality assessment of protoplast cultures.Plant methods · 2025Article
- Wild relatives to improve heat tolerance of cultivated quinoa (Chenopodium quinoa): pollen viability and grain number.Journal of experimental botany · 2025Article
- Reproductive Cold Stress in Contrasting Sorghum Genotypes: Is Pollen Fertility Really the Crucial Trait?Plant direct · 2025Article
- Screening methods for thermotolerance in pollen.Annals of botany · 2025Review
- An Analysis of Longan Honey from Taiwan and Thailand Using Flow Cytometry and Physicochemical Analysis.Foods (Basel, Switzerland) · 2024Article
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Integrated Microfluidics for Single-Cell Separation and On-Chip Analysis: Novel Applications and Recent Advances.Small science · 2024Article
- Deep learning-based high-throughput detection of in vitro germination to assess pollen viability from microscopic images.Journal of experimental botany · 2023Article
- Deductive automated pollen classification in environmental samples via exploratory deep learning and imaging flow cytometry.The New phytologist · 2023Article
- Development of a Novel Design of Microfluidic Impedance Cytometry for Improved Sensitivity and Cell Identification.ACS omega · 2023Article
- Evaluation of methods to assess the quality of cryopreserved Solanaceae pollen.Scientific reports · 2023Article
- Using Impedance Flow Cytometry for Rapid Viability Classification of Heat-Treated Bacteria.ACS omega · 2023Article
- Quantification of pollen viability inQuantitative plant biology · 2023Article
- Overcoming roadblocks forFrontiers in plant science · 2023Review
- Genomic Regions and Floral Traits Contributing to Low Temperature Tolerance at Young Microspore Stage in a Rice (Frontiers in plant science · 2022Article
- Low Salicylic Acid Level Improves Pollen Development Under Long-Term Mild Heat Conditions in Tomato.Frontiers in plant science · 2022Article
- Potential of Impedance Flow Cytometry to Assess the Viability and Quantity ofPlants (Basel, Switzerland) · 2021Article
- What is the future of electrical impedance spectroscopy in flow cytometry?Biomicrofluidics · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionAn efficient and reliable method to estimate plant cell viability, especially of pollen, is important for plant breeding research and plant production processes. Pollen quality is determined by classical methods, like staining techniques or in vitro pollen germination, each having disadvantages with respect to reliability, analysis speed, and species dependency. Analysing single cells based on their dielectric properties by impedance flow cytometry (IFC) has developed into a common method for cellular characterisation in microbiology and medicine during the last decade. The aim of this study is to demonstrate the potential of IFC in plant cell analysis with the focus on pollen.
methodDeveloping and mature pollen grains were analysed during their passage through a microfluidic chip to which radio frequencies of 0.5 to 12 MHz were applied. The acquired data provided information about the developmental stage, viability, and germination capacity. The biological relevance of the acquired IFC data was confirmed by classical staining methods, inactivation controls, as well as pollen germination assays.
resultsDifferent stages of developing pollen, dead, viable and germinating pollen populations could be detected and quantified by IFC. Pollen viability analysis by classical FDA staining showed a high correlation with IFC data. In parallel, pollen with active germination potential could be discriminated from the dead and the viable but non-germinating population.
conclusionThe presented data demonstrate that IFC is an efficient, label-free, reliable and non-destructive technique to analyse pollen quality in a species-independent manner.
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