ArticleNature protocols2016
Quantifying macromolecular interactions in living cells using FRET two-hybrid assays.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis.Mikrochimica acta · 2025Review
- Tailorable biosensors for real-time monitoring of stress distribution in soft biomaterials and living tissues.Nature communications · 2025Article
- Arrhythmia-associated calmodulin variants interact with KCNQ1 to confer aberrant membrane trafficking and function.PNAS nexus · 2023Article
- Critical contributions of pre-S1 shoulder and distal TRP box in DAG-activated TRPC6 channel by PIPScientific reports · 2022Article
- Cytosolic peptides encoding CaCommunications biology · 2022Article
- Interaction of human CRX and NRL in live HEK293T cells measured using fluorescence resonance energy transfer (FRET).Scientific reports · 2022Article
- Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells.Science advances · 2022Article
- Bak instead of Bax plays a key role in metformin-induced apoptosis s in HCT116 cells.Cell death discovery · 2021Article
- Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts.Journal of fluorescence · 2021Article
- An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel.BMC biology · 2021Article
- Probing ion channel macromolecular interactions using fluorescence resonance energy transfer.Methods in enzymology · 2021Article
- Article
- Stoichiometry and regulation network of Bcl-2 family complexes quantified by live-cell FRET assay.Cellular and molecular life sciences : CMLS · 2020Article
- Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.Scientific reports · 2019Article
- Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells.Scientific reports · 2019Article
- TPC2 polymorphisms associated with a hair pigmentation phenotype in humans result in gain of channel function by independent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Peripherin-2 and Rom-1 have opposing effects on rod outer segment targeting of retinitis pigmentosa-linked peripherin-2 mutants.Scientific reports · 2017Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Förster resonance energy transfer (FRET) is a versatile method for analyzing protein-protein interactions within living cells. This protocol describes a nondestructive live-cell FRET assay for robust quantification of relative binding affinities for protein-protein interactions. Unlike other approaches, our method correlates the measured FRET efficiencies to relative concentration of interacting proteins to determine binding isotherms while including collisional FRET corrections. We detail how to assemble and calibrate the equipment using experimental and theoretical procedures. A step-by-step protocol is given for sample preparation, data acquisition and analysis. The method uses relatively inexpensive and widely available equipment and can be performed with minimal training. Implementation of the imaging setup requires up to 1 week, and sample preparation takes ∼1-3 d. An individual FRET experiment, including control measurements, can be completed within 4-6 h, with data analysis requiring an additional 1-3 h.
Indexed as
Identifiers
27831569What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.